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Virplex® Acanthamoeba RealTime PCR Kit, 3-Plex, 24 Rxn
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Targets: 3-target multiplex (Acanthamoeba spp. + internal control)
Specifications:
| Application | qPCR, Molecular Diagnostics | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Lyophilized |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® Acanthamoeba RealTime PCR Kit is a multiplex real-time PCR assay designed for the detection of Acanthamoeba spp. nucleic acids in ophthalmological samples. This kit provides rapid and reliable identification of Acanthamoeba infections, which are a major cause of Acanthamoeba keratitis, a serious corneal disease often associated with contact lens use.
Key Features
- Multiplex PCR design – three targets detected in a single reaction tube.
- Format – 24 tests (3 individual strips of 8 wells).
- Broad qPCR compatibility – suitable for thermal cyclers with FAM, Cy5, HEX (VIC), and Texas Red detection channels.
- Endogenous control – ensures monitoring of inhibitors, nucleic acid integrity, and amplification set-up.
- Lyophilized reagents – master mix and positive control for long-term stability and simplified transport.
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Fast results – turnaround time of ~1 h 30 min.
- Research Use Only (RUO) – intended for laboratory and research applications.
Applications
- Detection of Acanthamoeba spp. in ophthalmology and vision-related research.
- Molecular diagnostics for Acanthamoeba keratitis in suspected eye infection cases.
- Epidemiological studies of protozoan eye infections.
- Laboratory training and method validation for ocular pathogen testing.
- Research use in parasitology and medical microbiology labs.
Product Details
| Property | Value |
|---|---|
| Product Name | Virplex® Acanthamoeba RealTime PCR Kit |
| Reference | RTPCR035-LP-R |
| Nº Tests | 24 (3 × 8-well strips) |
| Class | RUO – Research Use Only |
| Format | Multiplex real-time PCR kit |
| Sample Type | Ophthalmological specimens (e.g., corneal scrapings, swabs) |
| Targets | 3-target multiplex (Acanthamoeba spp. + internal control) |
| Internal Control | Endogenous control for sample validation |
| PCR Cycler Compatibility | FAM, Cy5, HEX (VIC), Texas Red |
| Time to Results | ~1 h 30 min |
| Reagents | Lyophilized master mix, positive control, negative control |
The Virplex® Acanthamoeba RealTime PCR Kit offers a fast, stable, and reliable solution for the detection of Acanthamoeba spp. in ocular samples. With its multiplex design, lyophilized reagents, and automation-ready workflow, it is an essential tool for ophthalmological research, outbreak monitoring, and molecular parasitology laboratories.