Rat Testosterone, T ELISA Kit

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Sensitivity: 0.01 ng/ml
Standard: 4.5 ng/ml
Sample Volume: 10ul
Wavelength: 450nm
Assay range: 0.1 ng/ml - 3.8ng/ml

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Specifications:
Application ELISA-Based Assays
Storage Temperature 2-8°C
Product Type Elisa Kit Forms Kit with Various components
Product Grade Molecular Biology

Quantitative Sandwich ELISA for Detection of Testosterone in Rat Serum, Plasma, and Biological Samples

The Rat Testosterone ELISA Kit is a high-sensitivity, quantitative sandwich ELISA designed for the in vitro determination of testosterone (T) levels in rat serum, plasma, cell culture supernatants, and tissue samples. Using a microplate pre-coated with a testosterone-specific antibody, this assay delivers reliable and reproducible results, suitable for use in endocrine, reproductive biology, toxicology, and preclinical research.

This kit provides a sensitivity of 0.01 ng/mL, with a detection range of 0.1–3.8 ng/mL, making it ideal for precise analysis of testosterone fluctuations in physiological and experimental settings.

Key Features & Benefits

  • High Sensitivity (0.01 ng/mL) – Enables detection of low testosterone levels in rat biological samples
  • Wide Assay Range (0.1–3.8 ng/mL) – Accommodates both physiological and pathological hormone levels
  • Validated Precision – Intra-assay CV <10%, Inter-assay CV <12%
  • Multi-Sample Compatibility – Suitable for serum, plasma, urine, cell culture media, and tissue homogenates
  • Complete Kit – Includes pre-coated microplate, standards, conjugates, buffers, and substrates
  • Storage at 2–8 °C – Stable for up to 6 months under recommended conditions
  • For Research Use Only (RUO) – Ideal for laboratory and preclinical applications

Kit Components (96 Tests)

ComponentVolume/Qty
MicroELISA strip plate (pre-coated)1 plate (8×12 wells)
Testosterone (T) Standard (4.5 ng/mL)0.5 mL × 1 bottle
Standard Diluent1.5 mL × 1 bottle
HRP-Conjugate Reagent6 mL × 1 bottle
Sample Diluent6 mL × 1 bottle
Chromogen Solution A (TMB A)6 mL × 1 bottle
Chromogen Solution B (TMB B)6 mL × 1 bottle
Stop Solution6 mL × 1 bottle
Wash Buffer (30× Concentrate)20 mL × 1 bottle
Closure Plate Membrane2 pieces
User Manual1
Sealed Storage Bags1

Sample Types

  • Rat serum
  • Plasma (EDTA, citrate)
  • Urine
  • Cell culture supernatants or lysates
  • Tissue homogenates

Assay Performance

AttributeValue
Assay Range0.1 – 3.8 ng/mL
Sensitivity0.01 ng/mL
Intra-assay CV<10%
Inter-assay CV<12%
Assay Time~2.5 hours
ReadingAbsorbance at 450 nm

Applications

🧬 Endocrinology Research – Monitor testosterone levels in hormonal studies

🧪 Reproductive Toxicology – Evaluate testicular function and hormone disruption

🧫 Preclinical Pharmacology – Assess drug effects on hormone levels in vivo

🧠 Behavioral Neuroscience – Study testosterone influence on animal behavior

🔬 Veterinary Research – Investigate hormonal biomarkers in animal models


The Rat Testosterone ELISA Kit (SL0668Ra) provides a sensitive, accurate, and easy-to-use platform for the quantification of testosterone in a wide range of biological fluids. Designed for research use only, this kit is ideal for scientists and labs conducting hormonal, toxicological, or reproductive studies in rat models.

  • Pack Size:   For 48 Tests For 96 Tests
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