Rat Testosterone, T ELISA Kit
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Sensitivity: 0.01 ng/ml
Standard: 4.5 ng/ml
Sample Volume: 10ul
Wavelength: 450nm
Assay range: 0.1 ng/ml - 3.8ng/ml
Specifications:
Application | ELISA-Based Assays | ||
Storage Temperature | 2-8°C | ||
Product Type | Elisa Kit | Forms | Kit with Various components |
Product Grade | Molecular Biology | ||
Quantitative Sandwich ELISA for Detection of Testosterone in Rat Serum, Plasma, and Biological Samples
The Rat Testosterone ELISA Kit is a high-sensitivity, quantitative sandwich ELISA designed for the in vitro determination of testosterone (T) levels in rat serum, plasma, cell culture supernatants, and tissue samples. Using a microplate pre-coated with a testosterone-specific antibody, this assay delivers reliable and reproducible results, suitable for use in endocrine, reproductive biology, toxicology, and preclinical research.
This kit provides a sensitivity of 0.01 ng/mL, with a detection range of 0.1–3.8 ng/mL, making it ideal for precise analysis of testosterone fluctuations in physiological and experimental settings.
Key Features & Benefits
- ✅ High Sensitivity (0.01 ng/mL) – Enables detection of low testosterone levels in rat biological samples
- ✅ Wide Assay Range (0.1–3.8 ng/mL) – Accommodates both physiological and pathological hormone levels
- ✅ Validated Precision – Intra-assay CV <10%, Inter-assay CV <12%
- ✅ Multi-Sample Compatibility – Suitable for serum, plasma, urine, cell culture media, and tissue homogenates
- ✅ Complete Kit – Includes pre-coated microplate, standards, conjugates, buffers, and substrates
- ✅ Storage at 2–8 °C – Stable for up to 6 months under recommended conditions
- ✅ For Research Use Only (RUO) – Ideal for laboratory and preclinical applications
Kit Components (96 Tests)
Component | Volume/Qty |
---|---|
MicroELISA strip plate (pre-coated) | 1 plate (8×12 wells) |
Testosterone (T) Standard (4.5 ng/mL) | 0.5 mL × 1 bottle |
Standard Diluent | 1.5 mL × 1 bottle |
HRP-Conjugate Reagent | 6 mL × 1 bottle |
Sample Diluent | 6 mL × 1 bottle |
Chromogen Solution A (TMB A) | 6 mL × 1 bottle |
Chromogen Solution B (TMB B) | 6 mL × 1 bottle |
Stop Solution | 6 mL × 1 bottle |
Wash Buffer (30× Concentrate) | 20 mL × 1 bottle |
Closure Plate Membrane | 2 pieces |
User Manual | 1 |
Sealed Storage Bags | 1 |
Sample Types
- ✅ Rat serum
- ✅ Plasma (EDTA, citrate)
- ✅ Urine
- ✅ Cell culture supernatants or lysates
- ✅ Tissue homogenates
Assay Performance
Attribute | Value |
---|---|
Assay Range | 0.1 – 3.8 ng/mL |
Sensitivity | 0.01 ng/mL |
Intra-assay CV | <10% |
Inter-assay CV | <12% |
Assay Time | ~2.5 hours |
Reading | Absorbance at 450 nm |
Applications
🧬 Endocrinology Research – Monitor testosterone levels in hormonal studies
🧪 Reproductive Toxicology – Evaluate testicular function and hormone disruption
🧫 Preclinical Pharmacology – Assess drug effects on hormone levels in vivo
🧠 Behavioral Neuroscience – Study testosterone influence on animal behavior
🔬 Veterinary Research – Investigate hormonal biomarkers in animal models
The Rat Testosterone ELISA Kit (SL0668Ra) provides a sensitive, accurate, and easy-to-use platform for the quantification of testosterone in a wide range of biological fluids. Designed for research use only, this kit is ideal for scientists and labs conducting hormonal, toxicological, or reproductive studies in rat models.
- Pack Size: For 48 Tests For 96 Tests