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Rapid Enzyme-Based Identification of Non-Fermenting and Oxidase-Positive Gram-Negative Bacilli
The Thermo Scientific™ RapID™ NF PLUS System is a rapid, manual microbial identification kit designed for the qualitative detection of over 70 clinically important oxidase-positive, Gram-negative bacilli, including Vibrio spp. and other medically relevant non-fermenters. Leveraging proprietary enzyme substrate technology, this micromethod delivers accurate results in just four hours, enabling timely and cost-effective diagnostics in clinical microbiology laboratories.
Key Features
- Fast Turnaround: Identify pathogens in 4 hours—supports urgent diagnostic decisions
- Manual Ease-of-Use: No oil overlays, pipetting, or long incubations required
- Broad Organism Coverage: Detects Pseudomonas, Vibrio, Aeromonas, and other oxidase-positive bacilli
- One-Step Inoculation: Simplifies preparation and saves valuable lab time
- Colorimetric Results: Clear, visible reactions reduce interpretation errors
- Data-Driven ID: Compatible with ERIC™ software or Code Compendium for confident reporting
- CE Marked: Certified for in vitro diagnostic use in regulated regions
Product Functionality
Each RapID NF PLUS panel contains a clear plastic tray with pre-filled wells of chromogenic and conventional substrates. The test organism is suspended in RapID Inoculation Fluid (R8325102) and dispensed into each cavity simultaneously. During the four-hour incubation, enzyme-substrate reactions produce visible color changes. In some wells, additional reagents such as RapID Spot Indole (R8309002) and RapID Nitrate A (R8309003) may be added to enhance visualization. The profile of positive/negative reactions is interpreted using Thermo Scientific’s database or ERIC™ software.
Applications
- Clinical Microbiology: Rapid ID of oxidase-positive, Gram-negative bacteria
- Hospital and Public Health Labs: Diagnosis of infections caused by Pseudomonas, Vibrio, Alcaligenes, Aeromonas, etc.
- Diagnostic Workflows: For isolates from human specimens where Enterobacteriaceae are excluded
- Research Use: Identification and characterization of environmental or clinical non-fermenters
Specifications Table
| Parameter | Details |
|---|---|
| Product Name | RapID™ NF PLUS System |
| Catalog Number | R8311005 |
| Detection Method | Enzyme-based substrate reaction |
| Detectable Organisms | >70 oxidase-positive, Gram-negative bacilli |
| Time to Result | 4 hours |
| Required Reagents | Spot Indole (R8309002), Nitrate A (R8309003), Inoc. Fluid (R8325102) |
| Packaging | 1 Box (20 Panels/Kit) |
| CE Marked | Yes |
| Intended Use | Manual, IVD—non-automated |
| Storage Conditions | 2°C to 8°C |
| Unit Size | Each |
Ordering Information
- Catalog Number: R8311005
- Kit Size: 20 Panels/Kit
- Storage Requirements: 2–8°C
- Regulatory Status: CE Marked for professional use
The RapID™ NF PLUS System offers a streamlined, accurate, and rapid approach to the identification of non-fermenting and oxidase-positive Gram-negative bacteria. Its intuitive design, fast processing time, and compatibility with comprehensive identification software make it a critical asset for modern clinical laboratories handling complex infections.
IFU: RapID NF Plus System
RapID NF Plus Microcode Worksheet
RapID NF Plus QC Report Form
Rapid Enzyme-Based Identification of Non-Fermenting and Oxidase-Positive Gram-Negative Bacilli
The Thermo Scientific™ RapID™ NF PLUS System is a rapid, manual microbial identification kit designed for the qualitative detection of over 70 clinically important oxidase-positive, Gram-negative bacilli, including Vibrio spp. and other medically relevant non-fermenters. Leveraging proprietary enzyme substrate technology, this micromethod delivers accurate results in just four hours, enabling timely and cost-effective diagnostics in clinical microbiology laboratories.
Key Features
- Fast Turnaround: Identify pathogens in 4 hours—supports urgent diagnostic decisions
- Manual Ease-of-Use: No oil overlays, pipetting, or long incubations required
- Broad Organism Coverage: Detects Pseudomonas, Vibrio, Aeromonas, and other oxidase-positive bacilli
- One-Step Inoculation: Simplifies preparation and saves valuable lab time
- Colorimetric Results: Clear, visible reactions reduce interpretation errors
- Data-Driven ID: Compatible with ERIC™ software or Code Compendium for confident reporting
- CE Marked: Certified for in vitro diagnostic use in regulated regions
Product Functionality
Each RapID NF PLUS panel contains a clear plastic tray with pre-filled wells of chromogenic and conventional substrates. The test organism is suspended in RapID Inoculation Fluid (R8325102) and dispensed into each cavity simultaneously. During the four-hour incubation, enzyme-substrate reactions produce visible color changes. In some wells, additional reagents such as RapID Spot Indole (R8309002) and RapID Nitrate A (R8309003) may be added to enhance visualization. The profile of positive/negative reactions is interpreted using Thermo Scientific’s database or ERIC™ software.
Applications
- Clinical Microbiology: Rapid ID of oxidase-positive, Gram-negative bacteria
- Hospital and Public Health Labs: Diagnosis of infections caused by Pseudomonas, Vibrio, Alcaligenes, Aeromonas, etc.
- Diagnostic Workflows: For isolates from human specimens where Enterobacteriaceae are excluded
- Research Use: Identification and characterization of environmental or clinical non-fermenters
Specifications Table
| Parameter | Details |
|---|---|
| Product Name | RapID™ NF PLUS System |
| Catalog Number | R8311005 |
| Detection Method | Enzyme-based substrate reaction |
| Detectable Organisms | >70 oxidase-positive, Gram-negative bacilli |
| Time to Result | 4 hours |
| Required Reagents | Spot Indole (R8309002), Nitrate A (R8309003), Inoc. Fluid (R8325102) |
| Packaging | 1 Box (20 Panels/Kit) |
| CE Marked | Yes |
| Intended Use | Manual, IVD—non-automated |
| Storage Conditions | 2°C to 8°C |
| Unit Size | Each |
Ordering Information
- Catalog Number: R8311005
- Kit Size: 20 Panels/Kit
- Storage Requirements: 2–8°C
- Regulatory Status: CE Marked for professional use
The RapID™ NF PLUS System offers a streamlined, accurate, and rapid approach to the identification of non-fermenting and oxidase-positive Gram-negative bacteria. Its intuitive design, fast processing time, and compatibility with comprehensive identification software make it a critical asset for modern clinical laboratories handling complex infections.
![[R4653047] Thermo Scientific™ RapID™ QC Set, Culti-Loops™ (RapID™ STAPH PLUS QC Set/4 (5/pk))](/web/image/product.product/43335/image_512/%5BR4653047%5D%20Thermo%20Scientific%E2%84%A2%20RapID%E2%84%A2%20QC%20Set%2C%20%20Culti-Loops%E2%84%A2%20%28RapID%E2%84%A2%20STAPH%20PLUS%20QC%20Set-4%20%285-pk%29%29?unique=96c5da2)
![[R8309002] Thermo Scientific™ RapID™ Spot Indole Reagent, 15 mL/Vial](/web/image/product.product/9630/image_512/%5BR8309002%5D%20Thermo%20Scientific%E2%84%A2%20RapID%E2%84%A2%20Spot%20Indole%20Reagent%2C%2015%20mL-Vial?unique=96c5da2)
![[R8309003] Thermo Scientific™ RapID™ Nitrate A Reagent, 15 mL/Vial](/web/image/product.product/37808/image_512/%5BR8309003%5D%20Thermo%20Scientific%E2%84%A2%20RapID%E2%84%A2%20Nitrate%20A%20Reagent%2C%2015%20mL-Vial?unique=96c5da2)
![[R8325102] Thermo Scientific™ RapID™ 1mL Inoculation Fluid (1 mL)](/web/image/product.product/37810/image_512/%5BR8325102%5D%20Thermo%20Scientific%E2%84%A2%20RapID%E2%84%A2%201mL%20Inoculation%20Fluid%20%281%20mL%29?unique=96c5da2)