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Thermo Scientific™ Middlebrook 7H10 Agar (Dehydrated)
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To isolate and cultivate Mycobacterium spp.
Specifications:
| Application | Clinical microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Remel | ||
| Product Grade | Microbiology grade | ||
Selective Culture Medium for the Isolation and Growth of Mycobacterium spp.
Thermo Scientific™ Remel™ Middlebrook 7H10 Agar (Dehydrated) is a specialized culture medium designed for the isolation and cultivation of Mycobacterium species, including Mycobacterium tuberculosis. This medium is based on the formulation developed by Middlebrook and Cohn in 1958, which enhances the rate and quality of mycobacterial growth using oleic acid and albumin supplementation. It is widely recognized in clinical and research settings for its selectivity, consistency, and ability to support luxuriant growth of Mycobacterium spp.
Key Features
- Selective for Mycobacterium: Contains malachite green dye to inhibit growth of non-mycobacterial contaminants
- Enhanced Mycobacterial Growth: Enriched with inorganic salts and capable of supporting fastidious species
- Dehydrated Format: Supplied in 500 g powder for customizable media preparation
- Validated Formulation: Based on Middlebrook and Cohn’s improved formula, promoting faster, more luxuriant growth
- Versatile Use: Suitable for qualitative procedures in clinical diagnostics, research, and public health microbiology
Applications
- Clinical Diagnostics: Primary isolation of Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) from patient specimens
- Public Health Laboratories: Tuberculosis surveillance and resistance monitoring
- Pharmaceutical Microbiology: Quality control and contamination assessment
- Research Institutions: Studies on pathogenic and environmental Mycobacterium spp.
Specifications Table
| Parameter | Details |
|---|---|
| Product Name | Middlebrook 7H10 Agar (Dehydrated) |
| Catalog Number | R453982 |
| Product Type | Culture Media (Agar) |
| Format | Dehydrated powder |
| Quantity | 500 g |
| Target Organisms | Mycobacterium spp. |
| Selective Agent | Malachite green dye |
| Usage | Qualitative procedures |
| Supplement Required | Requires ADC or OADC enrichment for full formulation |
| Storage Conditions | Store tightly closed in a cool, dry place |
| Compliance | For laboratory use only (non-IVD) |
Ordering Information
- Catalog Number: R453982
- Pack Size: 500 g
- Form: Dehydrated powder
- Use: Reconstitute with sterile water and enrichment supplement (e.g., ADC or OADC) before autoclaving
Thermo Scientific™ Middlebrook 7H10 Agar (Dehydrated) is a robust, selective medium ideal for the growth and differentiation of Mycobacterium species from clinical or environmental samples. Trusted by microbiologists worldwide, it offers reliable performance and compatibility with enrichment supplements for optimal diagnostic and research outcomes.