Thermo Scientific™ Remel™ Kovacs Indole Reagent

The Thermo Scientific™ Remel™ Kovacs Indole Reagent is a reliable, ready-to-use reagent designed to determine the ability of microorganisms, primarily Enterobacteriaceae, to split indole from tryptophan. This qualitative test aids in bacterial identification through a simple colorimetric reaction, providing consistent and accurate results.

Key Features:

1. Ready-to-Use:
   • Requires no additional preparation, allowing immediate use in laboratory workflows.
2. Accurate Identification:
   • Detects indole production by microorganisms through a clear, visible color change.
   • A pink-red ring at the broth-solvent interface indicates a positive result.
3. Proven Reagent Stability:
   • Substitution of isoamyl alcohol in the formulation enhances reagent stability and reliability.
4. High Sensitivity:
   • Designed to detect even small amounts of indole, ensuring precise differentiation of bacterial species.

Principle of the Test:

• Tryptophanase Enzyme:
  • Tryptophanase enzymes hydrolyze tryptophan, releasing indole.
  • Indole reacts with p-dimethylaminobenzaldehyde in the reagent, forming a pink-red compound.
• Result Interpretation:
  • Positive: Pink-red ring at the broth-solvent interface (indole present).
  • Negative: No color change or ring formation (indole absent).

Applications:

1. Clinical Diagnostics:
   • Differentiates Enterobacteriaceae and other indole-producing organisms for accurate bacterial identification.
2. Research:
   • Studies microbial enzymatic activity and metabolic pathways.
3. Quality Control:
   • Supports microbial differentiation in food, pharmaceutical, and environmental microbiology.

Specifications:

Reagent Composition:

Advantages:

1. Fast and Reliable:
   • Provides clear, easily interpretable results in a short time frame.
2. Ease of Use:
   • Pre-formulated and ready for immediate application.
3. Proven Accuracy:
   • Validated through quality control testing to ensure consistent performance.
4. Versatile Applications:
   • Suitable for clinical, research, and industrial microbiology labs.

Usage Instructions:

1. Grow the bacterial culture in tryptophan-containing broth.
2. Add Kovacs Indole Reagent dropwise to the surface of the broth.
3. Observe the interface for the formation of a pink-red ring (positive result).

Storage and Handling:

• Store at 2°C to 8°C in the original container to maintain reagent stability.
• Protect from direct light and avoid freezing.

Intended Use:

The Kovacs Indole Reagent is for in vitro diagnostic use and should be used by trained laboratory personnel to identify indole-producing microorganisms, aiding in bacterial differentiation and diagnostics.

References:

1. Kovacs, N.Z. 1928. Immunoforsch. 55:311.
2. Gadebusch, H.H. and S. Gabriel. 1956. Am. J. Clin. Pathol. 26:1373-1375.
3. MacFaddin, J.F. 1980. Biochemical Tests for Identification of Medical Bacteria. 2nd ed. Williams & Wilkins, Baltimore, MD.

Product Details:

The Thermo Scientific™ Remel™ Kovacs Indole Reagent is a trusted tool for rapid and accurate microbial identification. Its ready-to-use formulation, high sensitivity, and ease of interpretation make it indispensable for clinical diagnostics and research laboratories.