0
Thermo Scientific™ Invitrogen™ Qubit™ 4 Fluorometer, with WiFi
Catalog No :
CAS Number :
Brand :
Availability :
In Stock
Sample Volume: 1 to 20 µL
Excitation Wavelength:
Max ∼470 nm Blue LED
Max ∼635 nm Red LED
Specifications:
| Application | Fluorometry | Warranty | 1 Year |
| Storage Temperature | Room Temperature | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Qubit™ 4 Fluorometer with WiFi is a state-of-the-art device designed for accurate measurement of DNA, RNA, and protein concentration, along with RNA integrity and quality. It is widely used in molecular biology and genomic research, supported by highly sensitive Qubit assays and advanced features for data management and user convenience. Below is a detailed description:
Key Features:
- Accurate Quantitation:
- Measures DNA, RNA, protein concentrations, and RNA integrity (RNA IQ Assay) with high sensitivity.
- Enhanced accuracy for low-concentration samples, avoiding interference from contaminants.
- Rapid Results:
- DNA, RNA, and protein measurements in less than three seconds.
- RNA integrity analysis in less than five seconds per sample.
- Advanced Display and Interface:
- 5.7-inch color touchscreen with intuitive navigation.
- Graphical range indicators and on-board reagent calculators for ease of use.
- Data Management:
- WiFi-enabled for easy data transfer.
- Supports USB drive and cable transfers, with storage for up to 1,000 sample results.
- User-Friendly Design:
- Compact, space-saving footprint.
- Personalized settings with MyQubit software for custom assays.
- Multi-language support.
- Preloaded Assays:
- Includes pre-programmed assays for DNA, RNA, protein, and RNA IQ measurements.
Applications:
- Quantification of Biomolecules:
- DNA, RNA, and protein for qPCR, NGS, cDNA synthesis, and other molecular biology workflows.
- RNA Integrity Analysis:
- Evaluate intact vs. degraded RNA with RNA IQ assay.
- Sample Preparation:
- Assessment prior to downstream applications like sequencing and microarrays.
- Ion Sphere Particle (ISP) Quality Check:
- For sequencing runs using Ion Torrent platforms.
Specifications:
| Parameter | Details |
|---|---|
| Sample Volume | 1 to 20 µL |
| Excitation Wavelength | 470 nm (Blue LED), 635 nm (Red LED) |
| Data Transfer | WiFi, USB drive, USB cable |
| Languages Supported | English, French, Spanish, Italian, German, Simplified Chinese, Japanese |
| Measurement Speed | <3 seconds per sample |
| Screen Size | 5.7-inch color touchscreen |
| Storage Capacity | 1,000 sample results |
| Unit Size | 1 fluorometer |
| Compatibility | Qubit Assays, RNA IQ Kit |
Kit Contents:
- Qubit 4 Fluorometer
- WiFi Dongle
- USB Thumb Drive and Cable
- Universal Power Supply with 4 Plug Adapters
- Preloaded Software for Assays
Advantages Over UV Spectrophotometry:
- Higher sensitivity and accuracy at low concentrations.
- Specificity to target molecules through fluorescence detection.
- Minimal interference from salts, free nucleotides, or other contaminants.
The Invitrogen™ Qubit™ 4 Fluorometer is a powerful, versatile tool for quantitative molecular analysis, streamlining workflows and enhancing research precision.
Here is a comparative analysis of the Invitrogen™ Qubit™ 4 Fluorometer with other widely used methods and brands for nucleic acid and protein quantification, highlighting its unique advantages:
Comparison of Qubit™ 4 Fluorometer with Alternative Methods
| Feature | Qubit™ 4 Fluorometer | UV-Vis Spectrophotometry (e.g., NanoDrop) | BioTek Synergy (Microplate Reader) |
|---|---|---|---|
| Detection Mechanism | Fluorescence-based with target-specific dyes | Absorbance at 260/280 nm | Absorbance or fluorescence (multiplex) |
| Sensitivity | High sensitivity; detects as little as 1 ng/mL | Low sensitivity for dilute samples | Moderate sensitivity, dependent on setup |
| Specificity | High; dyes fluoresce only when bound to the target molecule (DNA, RNA, or protein) | Low; detects all nucleic acids, proteins, and contaminants | Moderate; depends on assay/dyes used |
| Quantification Range | Wide; detects from picogram to microgram levels | Limited for low concentrations (nanogram range) | Moderate; highly variable depending on assay |
| Ease of Use | User-friendly, touch-screen interface; simple protocols | Requires experience to interpret ratios | Advanced setup for multiplex assays |
| RNA Integrity Assessment | RNA IQ assay measures intact vs. degraded RNA | Not available | Not typically available |
| Sample Volume | 1–20 µL | 1–2 µL | 10–100 µL |
| Output Format | Concentration, purity, RNA quality | Absorbance ratios (260/280, 260/230) | Multiplexed data or single measurement |
| Data Management | WiFi, USB, and PC connectivity | Limited USB or manual logging | Advanced, often requires software setup |
| Time per Sample | <3 seconds (quantification); <5 seconds (RNA IQ) | Instant | 1–5 minutes/sample depending on setup |
| Compatibility | DNA, RNA, protein assays; RNA IQ for integrity | Primarily DNA/RNA; minimal protein specificity | DNA, RNA, protein, multiplex assays |
| Instrument Cost | Cost-effective | Moderate | Higher |
Comparison of Qubit™ 4 Fluorometer with Competitor Brands
| Feature | Invitrogen™ Qubit™ 4 Fluorometer | NanoDrop™ One/OneC Spectrophotometer | DeNovix DS-11 FX Spectrophotometer | Bio-Rad ChemiDoc MP Imaging System |
|---|---|---|---|---|
| Detection Mechanism | Fluorescence-based | UV-Vis absorbance | UV-Vis and fluorescence | Chemiluminescence, fluorescence, absorbance |
| Applications | DNA, RNA, protein quantification, RNA IQ | DNA, RNA quantification | DNA, RNA quantification, fluorescence assays | Imaging and quantification |
| Sensitivity | High; detects picograms | Low; limited for dilute samples | Moderate for fluorescence; high for absorbance | High sensitivity (picogram range) |
| RNA Integrity Assessment | RNA IQ Assay | Not available | Not available | Not available |
| Sample Volume | 1–20 µL | 1–2 µL | 1–2 µL | 10–50 µL |
| Multiplex Capability | Limited to one measurement per run | Not available | Multiplex fluorescence | Advanced multiplex (fluorescence/imaging) |
| Data Management | WiFi, USB, cloud storage | USB or direct PC connection | USB, touchscreen | USB, software |
| Consumable Costs | Moderate; specific assays required | None; direct measurement | Moderate for fluorescence assays | High; specialized reagents required |
Advantages of Qubit™ 4 Fluorometer Over Others
- RNA Integrity Assessment: Unique RNA IQ assay provides accurate intact vs. degraded RNA quantification
- Target Specificity: Fluorescent dyes minimize interference from contaminants, ensuring high specificity compared to UV-Vis methods like NanoDrop.
- Ease of Use: User-friendly touchscreen interface, WiFi-enabled data transfer, and compact design save time and space.
- Application Versatility: Compatible with DNA, RNA, and protein assays, as well as unique integrity metrics for RNA.
- Cost Efficiency: Mid-range pricing with low assay consumable costs compared to high-end imaging systems.
The Qubit™ 4 Fluorometer is ideal for researchers requiring precise, sensitive quantification and integrity analysis of biomolecules, offering superior performance over traditional UV-Vis methods and cost-effective solutions for advanced applications.
Citations and Use Cases:
- MRSA Sequencing: Rapid DNA extraction and sequencing directly from clinical plates for infection control intervention optimization (Blane et al., J Antimicrob Chemother).
- Water Microbiome Analysis: High-throughput sequencing of water microbiomes for microbial content determination (Brumfield et al., PLoS One).
- Dietary Microbiota Adaptation: Studying microbiota's response to dietary changes using NF-κB signaling (Cell Rep).
- Gene Expression in Tumor Analysis: Correlation of gene activity with immune response and tumor progression in Merkel cell carcinoma (J Immunother Cancer).
Product Specification not found.
Accessory Products
Add a Review
Customer Review
Showing
0
Of
0