CHROMagar™ Pseudomonas

For Detection and Isolation of Pseudomonas spp., Including P. aeruginosa

CHROMagar™ Pseudomonas is a selective chromogenic culture medium developed for the detection and isolation of Pseudomonas spp. from water, food, pharmaceutical, and environmental samples. Colonies of Pseudomonas spp. appear as intense blue-green, providing rapid and reliable visual identification—even in mixed cultures.

Designed to replace conventional media such as Cetrimide or King’s A, CHROMagar™ Pseudomonas is particularly useful for monitoring water quality, detecting psychrotrophic spoilage strains in refrigerated foods, and identifying opportunistic pathogens like P. aeruginosa.

🔹 Key Features & Benefits

• ✅ Distinct Colony Coloration:

  Organism	Colony Appearance
  Pseudomonas spp. (incl. P. aeruginosa)	Blue-green
  Enterobacteriaceae	Mauve to violet or inhibited

• ✅ Fast Results: Detectable within 24 hours of incubation.
• ✅ Flexible Testing Formats:
  • Compatible with membrane filtration for water testing (e.g., 100 mL samples)
  • Supports direct streaking and surface inoculation for food and environmental samples
• ✅ Easy to Use & Interpret:
  • One intensified, unique color for Pseudomonas colonies
  • Readable under normal lighting—no need for UV fluorescence or special indicators
• ✅ Clean Background: Suppresses interfering flora, improving accuracy in complex sample matrices.

🔹 Intended Use

CHROMagar™ Pseudomonas is intended for:

• Qualitative detection and enumeration of Pseudomonas spp. from:
  • Drinking water and recreational water (pools, spas, fountains)
  • Animal-derived foods (milk, cheese, meat, fish, eggs)
  • Respiratory samples (e.g., from cystic fibrosis patients)
  • Pharmaceuticals and cosmetics
  • Surfaces and equipment in cleanroom or production settings

⚠️ Not for clinical diagnostic purposes. Suspect colonies should be confirmed by identification methods (e.g., MALDI-TOF, oxidase test).

🔹 Public Health & Industrial Relevance

• Water Quality: P. aeruginosa is a key indicator organism for disinfection failure in public pools and spas.
• Food Safety: Psychrotrophic Pseudomonas spp. (e.g., P. fragi, P. lundensis, P. taetrolens) are responsible for spoilage of refrigerated foods—but rarely cause illness.
• Healthcare: P. aeruginosa is an opportunistic pathogen, particularly dangerous to immunocompromised individuals and those with cystic fibrosis.

📌 CHROMagar™ Pseudomonas supports early detection and control across public health, food safety, and pharmaceutical hygiene applications.

🔹 Test Procedure

1. Sample Types: Water, dairy, meats, respiratory secretions, surface swabs
2. Inoculation Methods:
   • Direct surface streaking
   • Membrane filtration (place membrane on agar)
   • Pour plate (if validated for food matrices)
3. Incubation:
   • Temperature: 30–37 °C (depending on protocol)
   • Time: 24–48 hours
   • Atmosphere: Aerobic
4. Interpretation:
   • Pseudomonas spp. = Intense blue-green colonies
   • Other bacteria = Mauve, violet, or inhibited

🔹 Ordering Information

🔹 Storage & Shelf Life

• Storage: 2–8 °C, dry, protected from light
• Shelf Life:
  • Prepared media: Use within validated timeframe
  • Powder: Up to 24 months when stored properly

🔹 Scientific Publications

• Wu et al., 2023 – Multidrug-resistant Pseudomonas aeruginosa in animal-derived foods
• Truong et al., 2022 – Evaluation of chromogenic media for P. aeruginosa in CF patient respiratory samples

CHROMagar™ Pseudomonas is a reliable and user-friendly chromogenic medium for the rapid detection and isolation of Pseudomonas spp. Its high specificity, vibrant blue-green colony differentiation, and compatibility with various testing methods make it a valuable solution for water monitoring, food quality control, and hygiene surveillance in pharmaceutical and healthcare environments.