Promega Wizard® Genomic DNA Purification Kit For 500 Bacteria Isolates, 300Ul (copy)

The Promega Wizard® Genomic DNA Purification Kit is a versatile, manual, solution-based DNA extraction system designed for isolating high-quality genomic DNA from a wide variety of sample types, including blood, animal and plant cells, bacteria, yeast, and tissue samples. The streamlined protocol is scalable, efficient, and produces DNA suitable for downstream applications such as PCR, restriction digestion, and hybridization assays.

Key Features:

1. Wide Sample Compatibility:
   • Extracts genomic DNA from a broad range of sources:
     • Blood: White blood cells and whole blood.
     • Cell Lines: Cultured cells from animal or plant origins.
     • Tissue: Animal and plant tissues.
     • Bacteria: Gram-positive and Gram-negative species.
     • Yeast: Efficient lysis and purification of genomic DNA.
2. Efficient Protocol:
   • Complete DNA purification in approximately 60 minutes.
   • Scalable for various input sample volumes.
3. High-Quality DNA Yield:
   • Produces DNA with high purity, suitable for:
     • PCR amplification.
     • Digestion with restriction enzymes.
     • Southern blotting, dot blotting, and other hybridization assays.
4. Solution-Based Method:
   • Simple, manual extraction method with no need for columns or beads.
   • Reduces the risk of contamination and sample loss during processing.
5. Comprehensive Kit:
   • Includes all necessary reagents for lysis, protein precipitation, DNA purification, and rehydration.

Kit Components:

Applications:

1. Molecular Biology Research:
   • Genomic DNA preparation for PCR, qPCR, and NGS workflows.
   • Suitable for genomic mapping and library construction.
2. Genotyping and Screening:
   • Restriction enzyme digestion and hybridization-based assays.
   • Southern blot analysis and slot/dot blot hybridizations.
3. Diagnostics and Forensics:
   • DNA extraction from blood and tissue samples for pathogen detection or forensic analysis.
4. Microbial Research:
   • DNA isolation from Gram-positive and Gram-negative bacteria for taxonomic or functional studies.
   • Yeast DNA extraction for molecular and genetic analyses.

Advantages:

1. Flexibility:
   • Scalable protocol to accommodate varying sample sizes and input volumes.
2. Simple and Reliable:
   • Solution-based manual extraction minimizes contamination and loss.
   • No need for specialized equipment, making it ideal for labs with limited resources.
3. High-Quality DNA:
   • Purified DNA is free of proteins, polysaccharides, and other contaminants.
   • Ready for use in sensitive downstream applications.
4. Time-Efficient:
   • Complete extraction within an hour, enhancing lab productivity.

Protocol Overview:

1. Cell Lysis:
   • Add Cell Lysis Solution to the sample to lyse cells and release nuclei.
2. Nuclei Lysis:
   • Treat with Nuclei Lysis Solution to break down nuclear membranes and release DNA.
3. Protein Precipitation:
   • Add Protein Precipitation Solution to precipitate proteins, leaving DNA in solution.
4. DNA Recovery:
   • Centrifuge and transfer the DNA-containing supernatant to a new tube.
   • Precipitate DNA with isopropanol and wash with ethanol.
5. DNA Rehydration:
   • Resuspend the purified DNA in DNA Rehydration Solution.

Specifications:

The Promega Wizard® Genomic DNA Purification Kit is a reliable and efficient solution for researchers requiring high-quality genomic DNA for a variety of molecular biology applications. With its simple, scalable, and versatile protocol, this kit is an indispensable tool for any molecular biology lab.