Pro-Lab Vision™ Monovalent Flagella Salmonella Antisera

The Pro-Lab Vision™ Monovalent Flagella Salmonella Antisera are highly specific reagents designed for the serological identification of Salmonella species based on their flagellar (H) antigens. These antisera target individual flagellar antigens and antigenic complexes, facilitating detailed serotyping as per the Kauffmann-White-Le Minor scheme, which is widely used in clinical, public health, and research laboratories for Salmonella identification.

The antisera are packaged in 3.0 mL dropper bottles for convenience, ensuring ease of use in diagnostic workflows. Each antiserum is produced from reference strains and rigorously absorbed to remove cross-reactions, offering reliable and accurate serotyping results.

Key Features

1. Extensive Coverage:
   • Includes antisera for a comprehensive range of flagellar (H) antigens, including individual factors (e.g., Factor 2, 5, 6) and antigenic complexes (e.g., E Complex, G Complex, z4 Complex).
2. High Specificity and Sensitivity:
   • Prepared using reference Salmonella strains, each antiserum is thoroughly absorbed to eliminate cross-reactivity.
3. Ready-to-Use:
   • Supplied in dropper bottles, ensuring precise dispensing and ease of use.
4. Compliance with Global Standards:
   • Follows the Kauffmann-White-Le Minor serotyping scheme for global consistency in Salmonella typing.
5. Batch-Specific Quality Control:
   • Each lot undergoes rigorous testing to ensure reliable results, validated with positive and negative control strains.
6. Versatile Applications:
   • Ideal for clinical, food safety, veterinary, public health, and research laboratories.

Applications and Use Cases

1. Clinical Diagnostics

• Purpose:
  • Serotype Salmonella isolates from patient samples for diagnosing enteric fever, gastroenteritis, and other Salmonella-related diseases.
• Use Case:
  • A hospital lab uses Factor 2 and Factor d antisera to serotype a clinical isolate of Salmonella Typhimurium obtained from a blood culture.

2. Food Safety Testing

• Purpose:
  • Identify Salmonella serotypes in contaminated food products during routine microbiological quality control.
• Use Case:
  • A food safety lab employs E Complex and z10 antisera to confirm the presence of Salmonella Enteritidis in poultry samples.

3. Public Health Surveillance

• Purpose:
  • Monitor and track Salmonella outbreaks by determining serotypes involved in foodborne illnesses.
• Use Case:
  • A public health lab uses L Complex and Factor 7 antisera to identify the serotype responsible for a Salmonella outbreak linked to raw vegetables.

4. Veterinary Microbiology

• Purpose:
  • Detect and serotype Salmonella in animals and animal feed to prevent zoonotic transmission.
• Use Case:
  • A veterinary lab uses G Complex and z15 antisera to serotype Salmonella isolates from livestock samples during routine screening.

5. Research and Academic Studies

• Purpose:
  • Facilitate detailed epidemiological studies and molecular research on Salmonella serotypes.
• Use Case:
  • Researchers use Factor eh, enx, enz15 antisera to serotype Salmonella strains for studying the diversity of flagellar antigens in environmental samples.

How to Use Pro-Lab Vision™ Monovalent Flagella Salmonella Antisera

1. Prepare the Sample:
   • Isolate the Salmonella strain on selective agar.
   • Prepare a suspension of the culture in sterile saline.
2. Antigen Testing:
   • Place a drop of the specific antiserum on a clean glass slide.
   • Add a loopful of the bacterial suspension to the antiserum drop.
   • Mix gently with a sterile loop.
3. Interpretation:
   • Observe for agglutination within 30 seconds to 1 minute.
   • Positive Result: Visible clumping indicates the presence of the corresponding H antigen.
   • Negative Result: No agglutination suggests the absence of the specific antigen.
4. Control Testing:
   • Always include positive and negative control strains to validate test results.

Advantages

1. Comprehensive Coverage:
   • Provides antisera for a wide range of Salmonella serotypes, including rare and complex flagellar antigens.
2. Accurate Serotyping:
   • High specificity eliminates cross-reactivity, ensuring reliable identification.
3. Ease of Use:
   • Ready-to-use dropper bottles simplify the workflow, reducing handling time.
4. Cost-Effective:
   • Allows precise serotyping without the need for advanced molecular methods.
5. Compliance with Standards:
   • Compatible with global standards for Salmonella typing, ensuring international reporting consistency.

Available Variants and Specific Applications

Storage and Stability

• Storage Conditions:
  • Store at 2–8°C in the original packaging.
• Shelf Life:
  • Refer to the expiration date indicated on the label.
• Stability:
  • Avoid repeated freeze-thaw cycles to maintain the antiserum’s integrity.

Precautions

• For in vitro diagnostic use only.
• Handle Salmonella cultures and antisera in accordance with biosafety guidelines.
• Wear personal protective equipment (PPE) during testing.

Why Choose Pro-Lab Vision™ Monovalent Flagella Salmonella Antisera?

1. Broad Range:
   • Extensive library of H antigen-specific antisera covers diverse Salmonella serotypes.
2. High Accuracy:
   • Specific absorption eliminates cross-reactions, providing reliable results.
3. Convenient Packaging:
   • Dropper bottles allow precise reagent dispensing, minimizing waste.
4. Global Compatibility:
   • Aligned with the internationally recognized Kauffmann-White-Le Minor scheme.
5. Versatile Applications:
   • Suitable for use in clinical, food safety, veterinary, and research laboratories.

References

1. Kauffmann-White-Le Minor Scheme: Standard for serological classification of Salmonella.
2. ISO 6579-1: Microbiology of food and animal feeding stuffs – Horizontal method for the detection of Salmonella spp.
3. CDC Guidelines: Laboratory identification and serotyping of Salmonella.

The Pro-Lab Vision™ Monovalent Flagella Salmonella Antisera are essential tools for accurate, reliable, and efficient Salmonella serotyping, enabling enhanced diagnostic and surveillance capabilities across multiple industries. Let me know if you need further customization!