Prolex™ Streptococcal Grouping Kit, 60 Tests

The Prolex™ Streptococcal Grouping Kit is a diagnostic tool designed for the rapid and accurate identification of Lancefield Streptococcal Groups A, B, C, D, F, and G. It utilizes acid extraction to isolate group-specific antigens, which are then detected using latex agglutination technology. This kit is a reliable, efficient solution for clinical microbiology laboratories in diagnosing streptococcal infections.

Key Features

1. Comprehensive Grouping:
   • Identifies Lancefield Groups A, B, C, D, F, and G, covering the most clinically relevant streptococcal pathogens.
2. Acid Extraction Method:
   • Ensures efficient extraction of group-specific antigens for accurate identification.
3. Latex Agglutination Technology:
   • Rapid visual detection of streptococcal groups through agglutination.
4. Complete Kit:
   • Includes all necessary reagents and materials, eliminating the need for additional purchases.
5. Ease of Use:
   • Straightforward protocol with minimal hands-on time, designed for use in clinical microbiology labs.
6. High Sensitivity and Specificity:
   • Ensures reliable differentiation of streptococcal groups, critical for appropriate clinical management.

Kit Contents

Testing Procedure

1. Sample Preparation:
   • Isolate streptococcal colonies from culture media (e.g., blood agar).
2. Antigen Extraction:
   • Use the Streptococcal Extraction Reagents 1, 2, and 3 in sequence to extract group-specific antigens from the bacterial sample.
3. Latex Agglutination:
   • Mix a drop of the extracted antigen with the corresponding Specific Latex Reagent on the Agglutination Card.
4. Interpretation:
   • Observe for visible agglutination within 1 minute:
     • Agglutination: Positive for the specific Lancefield group.
     • No Agglutination: Negative for that group.
5. Control Testing:
   • Use the Polyvalent Positive Control to validate reagent performance.

Applications

1. Clinical Diagnostics:
   • Rapid identification of pathogenic streptococcal groups involved in:
     • Pharyngitis (Group A Streptococcus)
     • Neonatal infections (Group B Streptococcus)
     • Skin and soft tissue infections
     • Endocarditis (Group D Streptococcus)
     • Other streptococcal infections (Groups C, F, and G)
2. Antimicrobial Stewardship:
   • Supports targeted antibiotic therapy by accurately identifying streptococcal species.
3. Infection Control:
   • Facilitates timely diagnosis and appropriate interventions to prevent the spread of streptococcal infections.

Advantages

• Speed:
  • Results available in less than 10 minutes, reducing diagnostic turnaround time.
• Comprehensive Group Coverage:
  • Detects six Lancefield groups, including the most clinically significant strains.
• User-Friendly:
  • Simplified procedure suitable for routine use in clinical laboratories.
• Cost-Effective:
  • Complete kit with all necessary components minimizes additional costs.
• High Reliability:
  • Validated reagents and controls ensure accurate and reproducible results.

Comparison with Other Kits

Conclusion

The Prolex™ Streptococcal Grouping Kit - Acid Extraction is a reliable and efficient diagnostic tool for identifying Lancefield groups A, B, C, D, F, and G. Its rapid turnaround time, ease of use, and comprehensive kit design make it an invaluable resource for clinical microbiology labs aiming to improve diagnostic accuracy and patient outcomes.