Pierce™ Coomassie Brilliant Blue Dye

Thermo Scientific Pierce Coomassie Brilliant Blue Dyes are commonly used reagents for protein detection in gel electrophoresis and protein quantification. Available in two variants, R-250 and G-250, these dyes offer versatile, sensitive, and reversible protein detection in various applications.

Key Features:

1. Two Variants for Different Applications:
   • Pierce Coomassie Brilliant Blue R-250: Red-tinted form, suitable for standard protein detection in gels.
   • Pierce Coomassie Brilliant Blue G-250: Green-tinted (colloidal) form, commonly used for Bradford-type protein assays and colloidal staining methods.
2. High Sensitivity:
   • Detects as little as 0.5 µg/cm² of protein in a gel matrix.
3. Reversible Staining:
   • Forms complexes with proteins through electrostatic interactions, which can be reversed under specific conditions.
4. Colorimetric Detection:
   • Dye changes from reddish-brown to bright blue upon binding to proteins, with an absorption maximum at 595 nm.
5. Flexible Applications:
   • Suitable for in-gel, in-solution, and in-blot protein detection.
6. Storage Stability:
   • Stable at room temperature, ensuring long-term usability.

Specifications:

Applications:

1. Protein Detection in Gels:
   • SDS-PAGE and native gel electrophoresis.
2. Protein Quantification:
   • Bradford assays using G-250 dye for precise measurements.
3. Blot Staining:
   • Visualization of proteins transferred to membranes.
4. Reversible Staining:
   • Temporary staining for downstream applications like mass spectrometry.

Comparison of R-250 and G-250 Dyes:

Advantages:

Catalog Numbers and Descriptions:

Handling and Storage:

• Storage Conditions: Store at room temperature for convenience.
• Follow proper safety and handling protocols when working with dyes.

For sensitive, reliable, and versatile protein detection, Pierce Coomassie Brilliant Blue Dyes are an excellent choice for your research needs.