Invitrogen™ alpha Amylase 1 Polyclonal Antibody, 1mL

The Invitrogen™ Alpha Amylase 1 Polyclonal Antibody is a high-quality, sheep-derived polyclonal antibody designed to detect alpha-amylase from human, porcine, and fungal sources. This antibody is well-suited for immunohistochemistry (IHC) on frozen sections, ELISA, and related research applications, offering broad specificity and reliability.

Key Features:

1. Specificity:
   • Recognizes human salivary alpha-amylase with cross-reactivity to porcine pancreatic amylase (50%) and weakly to fungal amylase (10%).
2. Host and Isotype:
   • Produced in sheep, providing a high-quality IgG polyclonal antibody.
3. Applications:
   • Validated for:
     • Immunohistochemistry (IHC): 1:20–1:200 (frozen sections).
     • ELISA: 1:500–1:3,000.
4. Affinity Purified:
   • Purified via affinity chromatography, ensuring high specificity and minimal background noise.
5. Liquid Format:
   • Supplied in PBS buffer, pH 7.2, with 0.09% sodium azide for preservation.
6. Broad Reactivity:
   • Reacts with alpha-amylase enzymes from human, pig, and fungi.

Product Specifications:

Applications:

1. Immunohistochemistry (IHC):
   • Detects alpha-amylase in frozen tissue sections for localization and distribution studies.
   • Dilution: 1:20–1:200.
2. ELISA:
   • Measures alpha-amylase levels in complex samples for quantitative or qualitative analysis.
   • Dilution: 1:500–1:3,000.

Use Cases:

1. Human Salivary Amylase Research:
   • Investigates the role of alpha-amylase in digestion and its regulatory pathways.
2. Porcine Amylase Analysis:
   • Supports studies on porcine pancreatic enzymes in comparative biology or industrial applications.
3. Fungal Amylase Detection:
   • Evaluates fungal amylase activity in biotechnology and food production processes.
4. Disease Research:
   • Explores alpha-amylase involvement in conditions such as salivary gland disorders, pancreatitis, or metabolic syndromes.
5. Industrial Enzyme Studies:
   • Assesses alpha-amylase efficacy in processes like breadmaking, brewing, and starch hydrolysis.

Advantages:

1. Broad Reactivity:
   • Detects alpha-amylase across multiple species, enabling versatile applications.
2. High Sensitivity:
   • Robust performance in ELISA and IHC, ensuring accurate detection of alpha-amylase.
3. Convenience:
   • Ready-to-use liquid format simplifies handling and application.
4. Reliable Storage:
   • Stable under recommended conditions, ensuring long-term usability.

Storage and Handling:

• Short-Term Storage: Store at 4°C for immediate use.
• Long-Term Storage: Store at -20°C to preserve antibody integrity; avoid repeated freeze/thaw cycles.
• Shipping: Shipped on wet ice for international orders to maintain quality during transit.

Target Information:

• Function:
  • Alpha-amylase catalyzes the hydrolysis of starch into sugars and plays a critical role in digestion and metabolic regulation.
• Gene Aliases:
  • AMY1, AMY1A, AMY1B, AMY1C.
• Applications:
  • Breadmaking, brewing, starch hydrolysis, and diagnostics of digestive health.

Product Details:

Recommended Secondary Antibodies:

The Invitrogen™ Alpha Amylase 1 Polyclonal Antibody is an essential tool for detecting alpha-amylase across human, porcine, and fungal samples. Its high sensitivity, broad reactivity, and validated applications in IHC and ELISA make it ideal for research in enzymology, metabolism, and industrial applications.