MERCK Yeast Peptone Dextrose Broth, suitable for microbiology, NutriSelect® Basic

YPD Broth (Yeast Peptone Dextrose) is a widely used, nonselective liquid medium for the cultivation and propagation of yeasts, particularly Saccharomyces cerevisiae, Pichia pastoris, and other yeast species. Its nutrient-rich formulation supports the growth of heterotrophic microorganisms, making it suitable for various applications in microbiology, molecular biology, and industrial fermentation.

Key Features:

1. Nutrient-Rich Composition:
   • Bacteriological Peptone (20 g/L): Provides nitrogenous nutrients.
   • Glucose (Dextrose) (20 g/L): Serves as the primary carbohydrate and energy source.
   • Yeast Extract (10 g/L): Supplies additional nitrogen, vitamins (including B-complex), and growth factors.
2. Nonselective Medium:
   • Supports a broad range of yeast species, including:
     • Saccharomyces spp.
     • Pichia spp.
     • Candida spp.
     • Zygosaccharomyces spp.
3. Cost-Effective Option:
   • Offered as NutriSelect® Basic, providing quality results at an economical price point.
4. Flexible Packaging:
   • Available in 250 g and 1 kg poly bottles to suit different laboratory scales.
5. Easy to Prepare:
   • Suspend 50 g in 1 L of distilled water, autoclave at 121°C for 15 minutes, and use.

Specifications:

Applications:

1. Molecular Biology:
   • Cultivate Saccharomyces cerevisiae for molecular cloning, protein expression, and gene editing studies.
2. Industrial Fermentation:
   • Supports yeast propagation for brewing, baking, and bioethanol production.
3. Microbial Genetics:
   • Provides an optimal medium for studying yeast genetics, including mutagenesis and transformation.
4. General Microbiology:
   • Use as a basal medium for routine yeast cultivation in research or quality control settings.

Preparation Instructions:

1. Weigh and Suspend:
   • Suspend 50 g of YPD Broth powder in 1 L of distilled water.
2. Dissolve:
   • Mix thoroughly to ensure complete dissolution.
3. Sterilize:
   • Autoclave at 121°C for 15 minutes.
4. Cool and Use:
   • Allow the medium to cool before inoculating with yeast or microorganisms.

Advantages:

1. Versatile Medium:
   • Suitable for cultivating various yeast strains across multiple applications.
2. High Nutrient Content:
   • Provides robust growth and supports high-density cultures.
3. Economical:
   • Cost-effective formulation for routine laboratory use.
4. Convenient Packaging:
   • Available in flexible pack sizes to meet different lab requirements.

Ordering Information:

Storage and Handling:

• Storage Conditions:
  • Store at room temperature in a dry environment.
• Shelf Life:
  • Refer to the product label for expiration date.

Related Products:

• YPD Agar: For solid yeast culture.
• Glucose (Dextrose): For supplementing microbial growth media.

The YPD Broth, NutriSelect® Basic, is an essential medium for laboratories studying yeasts and conducting molecular biology experiments. Its versatility, nutrient-rich composition, and cost-effectiveness make it a reliable choice for routine and specialized applications.