MyBioSource General Resistant Starch Assay Kit, 100 Assays

The MyBioSource General Resistant Starch Assay Kit is a colorimetric microplate-based assay designed for the detection and quantification of resistant starch (RS) content in various samples, including tissue extracts, food products, and agricultural products. This research-use-only kit provides reliable and reproducible results, making it a valuable tool for nutrition and agricultural research.

Key Features

1. Application:
   • Measures the content of resistant starch, a non-digestible carbohydrate that is essential for dietary and metabolic research.
   • Suitable for use with tissue extracts, food samples, and other agricultural products.
2. Assay Format:
   • Colorimetric microplate-based detection for ease of use and scalability.
   • Includes reagents and instructions to perform up to 100 assays.
3. Quantification:
   • Generates a standard curve for the accurate quantification of resistant starch content in test samples.
4. Storage and Stability:
   • The kit is stable for 6 months at 4°C.
5. Sample Types:
   • Compatible with a wide range of sample types, including:
     • Processed foods.
     • Raw agricultural products.
     • Biological extracts.
6. Research-Grade Tool:
   • Designed for research use only; not suitable for diagnostic or therapeutic applications.

Kit Components

Applications

1. Food Science:
   • Analyze resistant starch levels in processed and raw food products.
   • Evaluate the nutritional value of various food types.
2. Agricultural Research:
   • Quantify resistant starch in crops and agricultural by-products.
   • Study the impact of agricultural practices on starch composition.
3. Nutritional Research:
   • Investigate the role of resistant starch in human and animal diets.
   • Study the metabolic effects of resistant starch in laboratory settings.
4. Biological Research:
   • Analyze tissue extracts for resistant starch content to understand metabolic pathways.

Advantages

1. High Sensitivity and Specificity:
   • Detects resistant starch accurately, even at low concentrations.
2. Ease of Use:
   • Simple microplate-based workflow suitable for high-throughput applications.
3. Versatile Sample Compatibility:
   • Can be applied to a wide variety of sample types, including food and biological samples.
4. Reliable Results:
   • Reproducible results with the included standard curve for quantification.
5. Comprehensive Kit:
   • Includes all necessary reagents and components for performing 100 assays.

Assay Procedure (General Workflow)

1. Sample Preparation:
   • Prepare tissue extracts, food homogenates, or agricultural product samples as per the instructions.
2. Digestion:
   • Use Reagent A to digest non-resistant starch and remove interfering substances.
3. Reaction Setup:
   • Add Reagent B to selectively hydrolyze resistant starch into measurable components.
4. Color Development:
   • Incubate with the Colorimetric Substrate to produce a detectable signal.
5. Measurement:
   • Measure absorbance at the specified wavelength using a microplate reader.
6. Data Analysis:
   • Generate a standard curve and calculate resistant starch content in test samples.

Precautions

• For Research Use Only:
  • Not suitable for diagnostic, therapeutic, or prophylactic applications.
• Storage and Handling:
  • Store reagents at 4°C to maintain stability.
  • Briefly centrifuge vials to collect any liquid trapped in the caps during shipment.
• Safety Considerations:
  • Follow standard laboratory safety protocols during assay setup and execution.

Notes and Recommendations

1. Standard Curve:
   • Always include a standard curve with each run to ensure accurate quantification.
2. Sample Validation:
   • Test samples for compatibility with the assay reagents to ensure valid results.
3. Reagent Stability:
   • Avoid repeated freeze-thaw cycles of the reagents to maintain performance.
4. Troubleshooting:
   • Consult the manufacturer or technical support team if assay performance issues arise.

The MyBioSource General Resistant Starch Assay Kit is a reliable and versatile tool for quantifying resistant starch in a variety of research contexts. Its simple workflow, robust design, and compatibility with multiple sample types make it an essential kit for researchers in food science, agriculture, and nutrition.