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Thermo Scientific™ Oxoid™ Microbact™ TDA Reagent, 10mL
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Specifications:
| Application | Clinical microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Biochemical Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Microbact™ TDA Reagent (MB0180A) is a diagnostic reagent used to detect the presence of tryptophan deaminase (TDA) activity in bacterial isolates. The test is commonly employed as part of the Microbact™ Identification System to differentiate Proteus, Providencia, and Morganella species from other Enterobacteriaceae.
Key Features
- Rapid Identification:
- Provides results within 1–2 minutes, enabling quick differentiation of TDA-positive and TDA-negative organisms.
- Ready-to-Use Format:
- Supplied as a liquid reagent in a convenient 10mL dropper bottle, eliminating the need for additional preparation.
- Clear Color Reaction:
- Positive reaction: Red-brown color after adding the reagent.
- Negative reaction: No color change.
- Specific for TDA Activity:
- Reacts with tryptophan deaminase, an enzyme produced by certain Enterobacteriaceae.
- Broad Compatibility:
- Suitable for testing bacterial colonies as part of comprehensive identification workflows.
Applications
1. Clinical Microbiology
- Differentiation of Proteus, Providencia, and Morganella species from other Enterobacteriaceae in clinical specimens.
2. Food Microbiology
- Identification of foodborne pathogens, especially from the Enterobacteriaceae family.
3. Environmental Microbiology
- Testing environmental isolates for TDA activity as part of bacterial differentiation workflows.
4. Research Microbiology
- Studies involving the metabolic activity and enzyme profiles of bacterial species.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Tryptophan Deaminase (TDA) Reagent |
| Form | Liquid |
| Volume | 10mL |
| Applications | Clinical, food, environmental, and research microbiology |
| Reaction Time | 1–2 minutes |
| Unit Size | Each |
| Compatibility | Part of the Microbact™ Identification System |
Procedure
- Prepare Test Organism:
- Inoculate and grow a colony of the bacterial isolate on a suitable medium.
- Apply TDA Reagent:
- Add 1–2 drops of the Microbact™ TDA Reagent to the bacterial culture or test well.
- Observe Reaction:
- Wait for 1–2 minutes and observe for a color change.
- Interpret Results:
- Positive Reaction: Red-brown color indicates TDA activity.
- Negative Reaction: No color change.
Advantages
- Fast and Reliable: Provides accurate results in under 2 minutes.
- Easy to Use: Pre-prepared liquid reagent simplifies workflows.
- Clear Interpretation: Distinctive color change ensures easy differentiation of TDA-positive and TDA-negative organisms.
- Versatile Applications: Applicable across clinical, food, and environmental microbiology settings.
- Compact and Portable: Convenient 10mL bottle is suitable for field and laboratory use.
Quality Control
| Control | Expected Results |
|---|---|
| Positive Control | Proteus mirabilis – Red-brown reaction |
| Negative Control | Escherichia coli – No color change |
Storage and Stability
- Storage: Store at 2–8°C in the original container, away from direct sunlight.
- Shelf Life: Refer to the product label for the expiration date.
Packaging
- Product: Microbact™ TDA Reagent
- Catalog Number: MB0180A
- Volume: 10mL
The Thermo Scientific™ Oxoid™ Microbact™ TDA Reagent (MB0180A) is a fast, reliable, and easy-to-use reagent for detecting tryptophan deaminase activity in bacterial isolates. Its clear colorimetric results, compatibility with the Microbact™ Identification System, and broad application across clinical, food, and environmental microbiology make it an essential tool for bacterial differentiation workflows.