Mabtech Anti-human IFN-γ mAb (7-B6-1), biotin

Mabtech Anti-human IFN-γ mAb (7-B6-1), Biotin is a monoclonal antibody specifically designed for the detection of human IFN-γ in various immunoassays, including ELISpot, FluoroSpot, and ELISA. This biotinylated antibody is recommended for use in combination with capture antibodies, such as 1-D1K for human IFN-γ or MT126L for monkey IFN-γ, to enable accurate quantification and detection of IFN-γ levels in cell culture supernatants, blood samples, and other biological samples.

This product is ideal for high-throughput screening, cytokine release assays, and immune function studies, allowing researchers to evaluate immune responses and cellular activation.

Key Features:

1. Biotinylated Monoclonal Antibody:
   • The 7-B6-1 monoclonal antibody is biotinylated, enabling easy detection using streptavidin conjugates for immunoassays.
2. Specific Detection of Human IFN-γ:
   • This antibody specifically detects native and recombinant human IFN-γ, an essential cytokine involved in immune response, inflammation, and apoptosis.
3. Versatile for Multiple Assays:
   • Compatible with ELISpot, FluoroSpot, and ELISA techniques, offering flexibility for immune monitoring and cytokine profiling in cell-based assays.
4. Cross-reactivity:
   • Cross-reacts with IFN-γ from rhesus macaque and cynomolgus macaque, expanding the antibody's application to non-human primate research.
5. Purified and High-Quality:
   • The antibody is purified from in vitro cultures using Protein G affinity chromatography, ensuring high specificity and minimal non-specific binding.
6. Concentration:
   • Supplied at a concentration of 1 mg/mL, ensuring sufficient quantity for multiple assays and experiments.
7. Storage and Stability:
   • Store at 4-8°C short term or at -20°C for long-term storage. Avoid repeated freeze-thaw cycles to maintain optimal performance. The shelf life is 18 months from the date of receipt.

Specifications:

Applications:

1. ELISpot:
   • Used in ELISpot assays to quantify IFN-γ secretion from immune cells, providing insights into immune activation and cytokine release.
2. FluoroSpot:
   • Employed in FluoroSpot assays to detect IFN-γ production in T-cells or other immune cells, with the added benefit of fluorescent detection.
3. ELISA:
   • Suitable for ELISA assays to measure IFN-γ levels in cell culture supernatants, serum, and other biological fluids, used in cytokine profiling and immunology research.
4. Immunohistochemistry (IHC):
   • Can be used in paraffin sections for immunohistochemical detection of IFN-γ in tissue samples.
5. Flow Cytometry:
   • Can be employed for flow cytometry to analyze IFN-γ production by individual cells, allowing for high-throughput analysis of immune cell responses.
6. Cytokine Release Assays:
   • Useful in apoptosis, immune response, and drug screening assays to measure the IFN-γ secretion as an indicator of cellular activation.

Target Information:

IFN-γ (Interferon gamma) is a cytokine produced by T-cells and natural killer cells that plays a central role in immune response, inflammation, and apoptosis. It is primarily involved in the activation of macrophages, T-cell differentiation, and immune modulation. Caspase 8 and IFN-γ are important markers for apoptotic processes in response to stress or pathogen exposure. The 7-B6-1 antibody is a valuable tool for monitoring IFN-γ in immune studies and cancer research.

The Mabtech Anti-human IFN-γ mAb (7-B6-1), Biotin is an essential tool for immunoassays in immune research, providing high specificity and sensitive detection of IFN-γ. With its compatibility for ELISpot, FluoroSpot, and ELISA, this antibody is suitable for various applications, including cancer, neurodegenerative diseases, and infectious disease research.