Invitrogen™ PCNA Recombinant Rabbit Monoclonal Antibody (SY12-07), 100 µL

The Invitrogen™ PCNA Recombinant Rabbit Monoclonal Antibody (SY12-07) is a high-affinity antibody specifically designed for the detection of Proliferating Cell Nuclear Antigen (PCNA). This antibody is unconjugated, recombinant, and monoclonal, produced in vitro using HEK293 expression systems, ensuring high specificity, sensitivity, and batch-to-batch consistency.

PCNA is a crucial protein involved in DNA replication and repair, making it an essential marker for cell proliferation studies. This antibody has been validated for use in multiple applications, including Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC/IF), and Flow Cytometry (Flow).

Applications & Dilutions

The antibody has been tested and optimized for the following applications:

1. Western Blot (WB):
   • Recommended dilution: 1:5,000
   • Used to detect PCNA in cell or tissue lysates through gel electrophoresis and immunoblotting.
2. Immunohistochemistry (Paraffin) (IHC-P):
   • Recommended dilution: 1:1,000 to 1:2,000
   • Suitable for detecting PCNA in paraffin-embedded tissue sections.
3. Immunocytochemistry / Immunofluorescence (ICC/IF):
   • Recommended dilution: 1:2,000
   • Used for staining PCNA in cultured cells, allowing visualization under fluorescence microscopy.
4. Flow Cytometry (Flow):
   • Recommended dilution: 1:200
   • Ideal for analyzing PCNA expression in suspension cells via flow cytometry.

Product Specifications

Key Features & Benefits

1. High Specificity & Sensitivity:
   • Recognizes PCNA in human, mouse, and rat samples.
   • Targets an epitope within aa 88-137 of the PCNA protein.
2. Recombinant Monoclonal Antibody Advantages:
   • Produced using in vitro expression systems, eliminating animal-origin contaminants.
   • Ensures lot-to-lot consistency, reducing variability.
   • Provides high reproducibility and stability.
3. Versatile Applications:
   • Compatible with various immunodetection techniques including WB, IHC, ICC/IF, and Flow Cytometry.
   • Suitable for cancer research, cell cycle studies, and DNA damage repair analysis.
4. Stable Storage & Handling:
   • Short-term storage at 4°C (for immediate use).
   • Long-term storage at -20°C (to maintain antibody integrity).
   • Contains 40% glycerol, preventing freeze/thaw damage.

Scientific Background: PCNA (Proliferating Cell Nuclear Antigen)

PCNA (Polymerase delta auxiliary protein) is a critical nuclear protein involved in DNA replication and repair. It functions as a cofactor for DNA polymerase delta, forming a homotrimeric ring structure that encircles DNA to enhance processivity during leading-strand synthesis.

Functions of PCNA:

• Essential for DNA replication, repair, and recombination.
• Regulates cell cycle progression, particularly in G1 and S phases.
• Serves as a marker for cell proliferation, making it useful in cancer prognosis and tumor pathology studies.
• Involved in RAD6-dependent DNA repair pathways after DNA damage.
• Interacts with key proteins like CDK inhibitor p21, Fen1, XPG, and DNA methyltransferases.

Relevance in Cancer Research:

• Overexpression of PCNA is often associated with tumor growth and progression.
• Used as a biomarker for evaluating cell proliferation rates in various cancers.
• Its expression correlates with poor prognosis in certain malignancies.

Intended Use

• For Research Use Only (RUO).
• Not for diagnostic or therapeutic applications.
• Not for resale without authorization.

The Invitrogen™ PCNA Recombinant Rabbit Monoclonal Antibody (SY12-07) is an excellent choice for detecting PCNA in human, mouse, and rat samples. Its high sensitivity, specificity, and reproducibility make it an essential tool for cell proliferation studies, DNA repair research, and cancer diagnostics. With validation in multiple immunoassays, this antibody is a reliable and consistent reagent for molecular biology and biomedical research applications.