Invitrogen™ Mouse Caspase 8 Monoclonal Antibody (90A992)

The Invitrogen™ Mouse Caspase 8 Monoclonal Antibody (90A992) is a highly specific antibody designed to detect Caspase 8 in various biological samples, including human, mouse, and rat tissues. Caspase 8 is a key protease involved in programmed cell death (apoptosis), playing a central role in extrinsic apoptosis signaling pathways. This antibody is ideal for Western blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC/IF), flow cytometry (Flow), and Immunoprecipitation (IP) applications.

The antibody is a recombinant monoclonal from a mouse source and is unconjugated, offering flexibility for custom labeling and immunoassay applications. It specifically targets cleaved Caspase 8 and provides reliable detection of the active form of the enzyme during apoptosis.

Key Features:

1. Highly Specific to Caspase 8:
   • This mouse monoclonal antibody recognizes cleaved Caspase 8, a critical enzyme involved in extrinsic apoptosis. It plays a central role in the activation of downstream caspases, including Caspase 3, during apoptotic cell death.
2. Broad Species Reactivity:
   • The antibody is reactive to human, mouse, and rat, allowing for cross-species studies and making it suitable for a variety of experimental models.
3. Versatile Applications:
   • Western Blot (WB): For detecting Caspase 8 cleavage in protein samples.
   • Immunohistochemistry (IHC): Used for detecting Caspase 8 activation in tissue sections.
   • Immunocytochemistry (ICC/IF): To visualize Caspase 8 in live or fixed cells under fluorescence microscopy.
   • Flow Cytometry (Flow): To quantify Caspase 8 activation in large cell populations.
   • Immunoprecipitation (IP): For isolating Caspase 8 complexes in apoptosis research.
4. High Purity and Consistency:
   • Purified by Protein G affinity chromatography for minimal non-specific binding, ensuring reliable results across different experiments.
5. Unconjugated:
   • Unconjugated antibody for flexibility in custom labeling and integration with various fluorescent or colorimetric systems.
6. Recommended Dilution Range:
   • Western Blot (WB): 0.1-0.2 µg/mL
   • Immunohistochemistry (IHC): 1:10-1:50
   • Immunocytochemistry (ICC/IF): 1-2 µg/mL
   • Immunoprecipitation (IP): 1:100-1:300
7. Storage and Handling:
   • Store at 4°C short-term or -20°C long-term to maintain stability. The antibody contains 0.05% sodium azide as a preservative.
8. Clone 90A992:
   • This monoclonal antibody is a product of clone 90A992, ensuring high specificity for Caspase 8 and consistent performance in a variety of applications.

Specifications:

Applications:

1. Apoptosis Research:
   • Detect cleaved Caspase 8 in protein lysates from apoptotic cells to study programmed cell death.
2. Western Blotting (WB):
   • Used to identify Caspase 8 cleavage in protein extracts, providing insight into apoptosis and cell death mechanisms.
3. Immunohistochemistry (IHC):
   • Used for localizing Caspase 8 in tissue sections, enabling histological studies of apoptosis in disease tissues.
4. Immunocytochemistry (ICC/IF):
   • Visualizes Caspase 8 activation in live or fixed cells under fluorescence microscopy, ideal for cell-based studies.
5. Immunoprecipitation (IP):
   • Used to isolate Caspase 8 complexes and study protein-protein interactions in apoptosis pathways.
6. Flow Cytometry (Flow):
   • Quantifies Caspase 8 activation in cell populations, offering high-throughput apoptosis analysis.

Positive Control:

• Jurkat cells, treated with Cisplatin or other apoptotic inducers, to demonstrate Caspase 8 cleavage in apoptosis studies.

Target Information:

Caspase 8 (CASP8) is an important cysteine-aspartic acid protease involved in extrinsic apoptosis pathways. It is activated by death receptors like Fas and TNF receptors, leading to the cleavage of PARP1 and other substrates, committing the cell to programmed cell death. Caspase 8 is involved in initiating the apoptotic cascade, interacting with FADD (Fas-associated death domain), and is crucial for cytotoxic T-cell mediated cell death.

The Invitrogen™ Mouse Caspase 8 Monoclonal Antibody (123) is an invaluable tool for studying Caspase 8 activation and apoptosis across a range of experimental systems. With its high specificity, reliable performance, and compatibility with multiple techniques, this antibody is essential for cancer research, neurodegenerative disease studies, and drug development.