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Thermo Scientific™ Invitrogen™ HPV Type 18 E7 Monoclonal Antibody (718-15), 500 µg
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Specifications:
| Application | Western Blot, immunocytochemistry, Immunohistochemistry, Immunoprecipitation (IP) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ HPV Type 16/18 E7 Monoclonal Antibody (Clone 718-15) is a mouse IgG1 monoclonal antibody developed for the detection of E7 oncoproteins from human papillomavirus (HPV) types 16 and 18, two of the most oncogenic HPV strains. The antibody is validated for use in Western blot (WB) and ELISA, and is especially useful in cervical cancer and HPV-related head and neck cancer research.
This antibody targets the HPV18 E7 protein but demonstrates cross-reactivity to HPV16 E7, allowing researchers to study the two most high-risk HPV types with a single reagent. The E7 protein’s critical role in disrupting pRB (retinoblastoma protein) makes this antibody a valuable tool for studying HPV-driven carcinogenesis and viral-host interactions.
Applications & Validated Uses
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | Validated for HPV18 E7 |
| ELISA | Assay-dependent | Suitable for HPV18 E7 detection |
Note: Further assay optimization may be necessary for HPV16 E7 or low-abundance applications.
Key Features and Benefits
- Dual Reactivity: Reacts with both HPV16 and HPV18 E7 proteins, enabling broader experimental use.
- High Concentration Format: Supplied at 6.2 mg/mL for extended usability and flexibility in assay design.
- Protein G Purified: Ensures high purity and reduced background for sensitive applications.
- Stable Liquid Form: Ready-to-use format stored at 4°C with a PBS buffer and sodium azide preservative.
- Clone 718-15: Monoclonal antibody developed against HPV18 E7 oncoprotein with demonstrated virus specificity.
Specifications
| Property | Details |
|---|---|
| Clone | 718-15 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Antigen | HPV18 E7 oncoprotein |
| Cross-Reactivity | HPV16 E7 (reported) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 6.2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2 + 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic), Wet Ice (international) |
| RRID | AB_559528 |
Scientific Background
The E7 proteins of HPV types 16 and 18 are critical for viral oncogenesis. These small (~15 kDa) proteins inactivate pRB, disrupting cell cycle regulation and contributing to uncontrolled cell proliferation. Structurally, E7 contains a zinc finger motif and an LXCXE sequence essential for pRB binding. The disordered N-terminal and structured C-terminal regions of the E7 protein are evolutionarily conserved and key to its transforming function. Persistent infection with HPV16 or HPV18 is associated with ~70% of cervical and anogenital cancers, as well as a growing number of HPV-related head and neck cancers.
The Invitrogen™ HPV Type 16/18 E7 Monoclonal Antibody (Clone 718-15) is a versatile and high-affinity antibody developed for the detection and characterization of HPV E7 proteins, especially those from the high-risk types HPV16 and HPV18. Suitable for Western blot and ELISA applications, this antibody is an essential reagent for virology and cancer research focusing on HPV-mediated transformation and immune detection of viral oncoproteins.