Invitrogen™ Mouse Caspase 9 Monoclonal Antibody (LAP6 96-2-22), 200 µL

The Invitrogen™ Mouse Caspase 9 Monoclonal Antibody (LAP6 96-2-22) is designed for detecting Caspase 9 in human, mouse, and rat samples. Caspase 9 plays a central role in apoptosis, specifically in the intrinsic (mitochondrial) pathway, and is responsible for activating downstream caspases such as Caspase 3 and Caspase 7. This antibody is highly specific to Caspase 9 and is widely used in Western blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC/IF), flow cytometry (Flow), and immunoprecipitation (IP) applications.

The monoclonal antibody (LAP6 96-2-22) recognizes the pro-form and active form of Caspase 9 and has been validated in cell-based assays, particularly in studies related to programmed cell death and apoptosis research.

Key Features:

1. Specific to Caspase 9:
   • This antibody is specific to Caspase 9, an essential enzyme in the apoptotic pathway, making it a valuable tool for cell death studies.
2. Applications:
   • Suitable for Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC/IF), flow cytometry (Flow), and Immunoprecipitation (IP), allowing flexibility across experimental platforms.
3. Species Reactivity:
   • Reactive with Human, Mouse, and Rat samples, making it ideal for cross-species apoptosis studies.
4. Dilution Range:
   • Western Blot (WB): 0.1-0.2 µg/mL
   • Immunohistochemistry (IHC): 1:10-1:50
   • Immunocytochemistry (ICC/IF): 1-2 µg/mL
   • Immunoprecipitation (IP): 1:100-1:300
5. Purification:
   • The antibody is purified by Protein A/G affinity chromatography, ensuring high purity and minimal non-specific binding.
6. Unconjugated:
   • The antibody is unconjugated, providing flexibility for custom labeling and compatibility with various detection systems.
7. Storage:
   • Store at 4°C short term or -20°C long term with 0.02% sodium azide as a preservative to maintain stability.

Specifications:

Applications:

1. Apoptosis Research:
   • Detect cleaved Caspase 9 in apoptotic cells to study programmed cell death and apoptosis mechanisms.
2. Western Blotting (WB):
   • Caspase 9 antibody for detecting pro- and cleaved Caspase 9 in protein samples, providing insights into cell death and apoptosis in response to various stimuli.
3. Immunohistochemistry (IHC):
   • Visualize Caspase 9 activation in tissue sections and study apoptosis in diseased tissues.
4. Immunocytochemistry (ICC/IF):
   • Staining for Caspase 9 in live or fixed cells under fluorescence microscopy to monitor apoptotic activity.
5. Immunoprecipitation (IP):
   • Used for isolating Caspase 9 protein complexes in apoptotic signaling pathways.
6. Flow Cytometry (Flow):
   • Quantify Caspase 9 cleavage in cell populations, allowing for high-throughput apoptosis analysis.

Positive Control:

• HEK 293 whole cell lysate or Cisplatin-treated Jurkat cells to demonstrate Caspase 9 activation in apoptosis.

Target Information:

Caspase 9 (CASP9) is a key cysteine-aspartic acid protease involved in the intrinsic apoptosis pathway. It is activated by the Apoptotic Protease Activating Factor 1 (Apaf-1) and subsequently activates Caspases 3 and 7, leading to the execution phase of apoptosis. Caspase 9 is involved in the mitochondrial pathway of apoptosis and plays a pivotal role in cell death induced by DNA damage, cellular stress, and oxidative damage.

The Invitrogen™ Mouse Caspase 9 Monoclonal Antibody (LAP6 96-2-22) is a highly reliable and versatile tool for studying apoptosis. With its specificity for Caspase 9, it is ideal for Western blotting, immunohistochemistry, immunocytochemistry, flow cytometry, and immunoprecipitation applications. Whether studying cancer, neurodegenerative diseases, or drug-induced apoptosis, this antibody is essential for understanding apoptotic cell signaling and cell death mechanisms.