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Liofilchem™ MTS™ Ertapenem ETP 0.002-32, MIC Test strips
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Specifications:
| Application | Clinical microbiology |
| Storage Temperature | -20°C |
| Product Type | MIC Strips |
| Product Brand | Liofilchem |
| Product Grade | Microbiology grade |
The Liofilchem™ MTS™ Ertapenem MIC Test Strips (ETP 0.002–32) are gradient diffusion strips designed to determine the minimum inhibitory concentration (MIC) of ertapenem, a carbapenem antibiotic. These strips provide a reliable phenotypic method for testing Gram-negative bacterial resistance, particularly in detecting carbapenem-resistant Enterobacterales (CRE) and other resistant pathogens.
Key Features
- Wide MIC Range: 0.002–32 µg/mL, allowing precise determination of susceptibility or resistance.
- Accurate Gradient Diffusion: Produces sharp and well-defined ellipses for easy endpoint interpretation.
- Target Pathogens: Effective for testing Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii.
- Regulatory Standards: Fully compliant with CLSI and EUCAST antimicrobial susceptibility testing (AST) guidelines.
- Ease of Use: Compatible with standard laboratory techniques using Mueller-Hinton Agar.
Intended Use
- Detection of Carbapenem Resistance: Used for detecting resistance mechanisms in multidrug-resistant organisms.
- Therapeutic Decision Support: Guides empirical and targeted therapy for serious infections.
- Epidemiological Studies: Supports monitoring and surveillance of antimicrobial resistance (AMR).
Testing Procedure
1. Sample Preparation
- Prepare a bacterial suspension in sterile saline or broth equivalent to 0.5 McFarland standard.
- Spread the suspension evenly on a Mueller-Hinton Agar plate using a sterile swab.
2. Placement of MIC Strips
- Place the Liofilchem™ MTS™ Ertapenem strip on the agar surface with the gradient side facing upward.
- Ensure the strip adheres properly and avoid air bubbles beneath the strip.
3. Incubation
- Incubate the plate at 35–37°C for 16–20 hours under aerobic conditions.
4. Reading the Results
- Observe the elliptical inhibition zone around the strip.
- Determine the MIC as the point where the ellipse intersects the strip scale.
- Interpret results using CLSI or EUCAST breakpoint tables.
Results Interpretation
| MIC Range (µg/mL) | Interpretation (CLSI M100) | Interpretation (EUCAST) |
|---|---|---|
| ≤ 0.5 | Susceptible | Susceptible |
| 1 | Intermediate | Intermediate |
| ≥ 2 | Resistant | Resistant |
Quality Control
| Control Strains | Expected MIC Range (µg/mL) |
|---|---|
| Escherichia coli ATCC® 25922 | 0.004–0.016 |
| Klebsiella pneumoniae ATCC® 700603 | 0.25–1 |
| Pseudomonas aeruginosa ATCC® 27853 | 0.5–2 |
Technical Specifications
| Feature | Details |
|---|---|
| Active Agent | Ertapenem |
| MIC Range | 0.002–32 µg/mL |
| Recommended Media | Mueller-Hinton Agar |
| Incubation Conditions | 35–37°C for 16–20 hours |
| Storage Conditions | -20°C for long-term, 2–8°C for short-term |
| Shelf-Life | As indicated on packaging |
Advantages
| Feature | Benefit |
|---|---|
| Wide MIC Range | Provides accurate results across susceptibility profiles. |
| Sharp Inhibition Zones | Ensures easy and reproducible endpoint determination. |
| Regulatory Compliance | Meets CLSI and EUCAST standards for global applicability. |
| Cost-Effective | Offers a reliable alternative to molecular testing. |
| High Sensitivity and Specificity | Accurately detects low-level resistance. |
Comparisons
| Method | Advantages | Limitations |
|---|---|---|
| Liofilchem MIC Strips | High accuracy, phenotypic detection | Requires trained personnel for interpretation |
| Disk Diffusion | Simple and low-cost | Less precise than MIC determination |
| Automated Systems | Rapid and automated results | Expensive equipment required |
| Molecular Testing | Gene-specific detection | Cannot assess phenotypic expression |
Standard Reference Data
- CLSI M100 Guidelines: Standards for susceptibility testing breakpoints.
- EUCAST Recommendations: Breakpoints for carbapenem resistance.
- ISO Standards: Quality benchmarks for laboratory procedures.
Applications
- Hospital Infection Control: Detection of carbapenem resistance in clinical settings.
- AMR Surveillance Programs: Tracks resistance trends regionally and globally.
- Therapeutic Guidance: Supports optimal antibiotic selection in critical infections.
Conclusion
The Liofilchem™ MTS™ Ertapenem (ETP) 0.002–32 MIC Test Strips are highly effective diagnostic tools for detecting ertapenem resistance in Gram-negative bacteria. Their precision, compliance with international standards, and versatility make them essential for laboratories combating antimicrobial resistance.
- Pack Size: 100/pk 10/pk 30/pk