Liofilchem™ MTS™ Ampicillin-sulbactam SAM 0.016/4-256/4, MIC Test strips

Liofilchem™ MTS™ Ampicillin-Sulbactam (SAM) MIC Test Strips are advanced antimicrobial gradient diffusion tools used to determine the Minimum Inhibitory Concentration (MIC) of ampicillin-sulbactam, a beta-lactam and beta-lactamase inhibitor combination. These strips are specifically designed to assess the susceptibility of bacterial pathogens to the combination of ampicillin and sulbactam in a 2:1 ratio, supporting precise and reliable antimicrobial susceptibility testing (AST).

Key Features

• Dual-Drug Combination: Test strips impregnated with a gradient concentration of ampicillin and sulbactam in a fixed 2:1 ratio.
• MIC Range: Detects MIC values from 0.016/4 µg/mL to 256/4 µg/mL, suitable for a wide variety of clinical pathogens.
• High Precision: Ensures accurate determination of the MIC values, supporting clinical decisions for treatment of infections caused by beta-lactamase-producing bacteria.
• Easy-to-Use: Designed for seamless application in clinical microbiology laboratories with results interpretable as a precise MIC value.
• Breakpoints Compatible: Results are compatible with CLSI and EUCAST guidelines for antimicrobial susceptibility testing.

Applications

1. Clinical Microbiology:
   • Determining susceptibility of Gram-negative bacteria such as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter spp..
   • Testing against beta-lactamase-producing bacteria that are resistant to standalone beta-lactams.
2. Antimicrobial Resistance Monitoring:
   • Supporting surveillance programs to track resistance patterns in hospital and community-acquired infections.
3. Research and Development:
   • Used in pharmaceutical studies for evaluating combination therapies involving ampicillin-sulbactam.

Test Procedure

1. Preparation:
   • Prepare Mueller-Hinton agar (MHA) or another suitable medium following standard protocols.
   • Adjust the bacterial suspension to 0.5 McFarland standard.
2. Application:
   • Apply the MTS™ Ampicillin-Sulbactam strip onto the inoculated agar surface using sterile forceps.
   • Ensure complete contact between the strip and agar.
3. Incubation:
   • Incubate plates at 35 ± 2°C in an aerobic environment for 16-20 hours.
4. Result Interpretation:
   • Observe the intersection of the inhibition ellipse with the MIC scale on the strip.
   • Report results as the MIC of the ampicillin-sulbactam combination in µg/mL.

Quality Control

The performance of MTS™ Ampicillin-Sulbactam strips is validated using the following control organisms:

• Escherichia coli ATCC® 25922 – Expected MIC within quality control ranges.
• Klebsiella pneumoniae ATCC® 700603 – Beta-lactamase positive control.
• Pseudomonas aeruginosa ATCC® 27853 – Beta-lactamase negative control.

Storage and Stability

• Store at 2–8°C in a dry environment, protected from light.
• Use before the expiration date printed on the packaging.

Ordering Information

Product	Format	Catalog Number
MTS™ Ampicillin-sulbactam SAM 0.016/4-256/4	30 strips per pack	92181
MTS™ Ampicillin-sulbactam SAM 0.016/4-256/4	10 Strips per pack	921811
MTS™ Ampicillin-sulbactam SAM 0.016/4-256/4	100 strips per pack	921810

Precautions

• For in vitro diagnostic use (IVD) only.
• Intended for use by trained laboratory professionals.
• Ensure proper disposal of used strips and culture plates in accordance with institutional and local biosafety guidelines.

Regulatory Compliance

• Meets the standards set by CLSI, EUCAST, and ISO 11133 for quality control in antimicrobial susceptibility testing.

References

1. Clinical and Laboratory Standards Institute (CLSI) guidelines.
2. EUCAST breakpoint interpretations.
3. Manufacturer’s technical documentation for Liofilchem MTS™ strips.

Key Benefits

• Comprehensive testing for beta-lactam/beta-lactamase inhibitor resistance.
• Simple and reliable methodology for MIC determination.
• Supports rapid and informed clinical decision-making in antimicrobial therapy.