Liofilchem® Amoxicillin Antimicrobial Susceptibility Discs, 5µg, 250 Discs/Pack

Liofilchem® Amoxicillin Antimicrobial Susceptibility Discs are standardized discs impregnated with defined concentrations of amoxicillin (2 µg, 10 µg, 25 µg, and 30 µg) used for antimicrobial susceptibility testing of bacteria. These discs provide a reliable and accurate method for determining bacterial sensitivity or resistance to amoxicillin, a broad-spectrum penicillin antibiotic effective against a range of Gram-positive and Gram-negative bacteria.

Product Overview:

Amoxicillin, a beta-lactam antibiotic, works by inhibiting bacterial cell wall synthesis, making it effective against susceptible organisms. Liofilchem® antimicrobial susceptibility discs ensure consistent and reproducible results in clinical and research laboratories.

Key Features:

1. Standardized Drug Concentrations: Available in 2 µg, 10 µg, 25 µg, and 30 µg concentrations to suit varying testing requirements.
2. Quality Assurance: Manufactured in compliance with CLSI (Clinical and Laboratory Standards Institute) and EUCAST (European Committee on Antimicrobial Susceptibility Testing) guidelines.
3. Broad Spectrum of Activity: Effective against common pathogens, including:
   • Gram-positive bacteria: Streptococcus spp., Enterococcus spp., Staphylococcus spp.
   • Gram-negative bacteria: Escherichia coli, Haemophilus influenzae, and others.
4. High Precision: The impregnated discs ensure even diffusion of the antibiotic for accurate zone diameter measurements.
5. Convenient Packaging: Discs are stored in cartridges within desiccated, airtight containers to maintain potency and stability.
6. Customizable Use: Compatible with Mueller-Hinton Agar and other recommended culture media.

Applications:

• Determining bacterial susceptibility to amoxicillin in clinical diagnostics.
• Evaluating resistance patterns in community-acquired and hospital-acquired infections.
• Monitoring emerging antimicrobial resistance in bacterial pathogens.
• Research and development of new beta-lactamase inhibitors and antibiotic formulations.

Technical Specifications:

1. Drug: Amoxicillin (AML)
2. Concentrations: 2 µg, 10 µg, 25 µg, 30 µg
3. Disc Diameter: 6 mm
4. Recommended Medium: Mueller-Hinton Agar
5. Storage Conditions: Store at 2-8°C in the original airtight container.
6. Shelf Life: Refer to the product label for expiry date; typically valid for up to 2 years from the date of manufacture.

Test Procedure:

1. Preparation:
   • Prepare a standardized bacterial suspension equivalent to 0.5 McFarland turbidity standard.
   • Inoculate the suspension onto a Mueller-Hinton Agar plate evenly.
2. Application:
   • Place the appropriate Liofilchem® Amoxicillin Disc onto the agar surface using a sterile forceps or automated dispenser.
3. Incubation:
   • Incubate the plate at 35 ± 2°C for 16-18 hours in an aerobic atmosphere.
4. Interpretation:
   • Measure the zone of inhibition around the disc using a ruler or caliper.
   • Compare the zone diameter with CLSI/EUCAST breakpoint tables to determine susceptibility or resistance.

Quality Control:

Liofilchem® Amoxicillin AML Discs are tested against standard quality control strains to ensure accuracy and reproducibility:

• Escherichia coli ATCC® 25922
• Staphylococcus aureus ATCC® 25923
• Haemophilus influenzae ATCC® 49247

Packaging Options:

• Cartridges containing 50 discs each.
• Packs of 5 cartridges for a total of 250 discs.
• Desiccated and sealed to maintain potency.

Regulatory Compliance:

• Certified for in vitro diagnostic use (IVD).
• Manufactured according to ISO 9001 and ISO 13485 standards.
• Meets CLSI and EUCAST antimicrobial susceptibility testing guidelines.

Warnings and Precautions:

1. For laboratory use only; not intended for human or veterinary therapeutic purposes.
2. Follow biosafety guidelines for handling infectious agents.
3. Use within the expiry date for optimal results.

Ordering Information: