Thermo Scientific™ Fast DNA End Repair Kit, 50 Rxns

The Thermo Scientific™ Fast DNA End Repair Kit is a rapid and reliable solution for converting fragmented or overhang-containing DNA into blunt-ended, 5′-phosphorylated fragments, ready for blunt-end ligation. In just 5 minutes, this kit efficiently performs both end polishing and phosphorylation, using a pre-formulated mix that minimizes hands-on time and variability.

The enzyme mix includes T4 DNA Polymerase, Klenow Fragment, and T4 Polynucleotide Kinase (PNK), while the 10X Reaction Mix contains ATP and dNTPs in an optimized buffer.

Key Features and Benefits

• Rapid Protocol – Blunting and phosphorylation completed in only 5 minutes
• All-in-One Reaction – Simultaneous DNA polishing and 5′-phosphate addition
• Pre-Mixed Reagents – Reduced pipetting, lower error rates
• High Compatibility – Works with fragmented genomic DNA, restriction-digested plasmids, PCR products, and cDNA
• Reliable Performance – Suitable for 0.5–5 µg DNA input

Specifications

Applications

• Blunting and phosphorylation of:
  • Fragmented genomic DNA (e.g., sonicated, nebulized)
  • Restriction enzyme-digested plasmids
  • Double-stranded cDNA
  • PCR products with 3′ dA overhangs

Kit Contents

• End Repair Enzyme Mix
• 10X End Repair Reaction Mix (Buffer + ATP + dNTPs)

The Thermo Scientific™ Fast DNA End Repair Kit offers a highly efficient, 5-minute workflow for converting DNA into ligation-ready blunt-end fragments. Its enzyme mix and optimized buffer system simplify DNA end repair for molecular cloning and synthetic biology applications, making it ideal for fast-paced research environments.