Invitrogen™ Collibri™ Library Amplification Master Mix

The Invitrogen™ Collibri™ Library Amplification Master Mix is a high-fidelity, ready-to-use PCR solution specifically designed for efficient amplification of next-generation sequencing (NGS) libraries. Optimized for Illumina-compatible library preparation workflows, this master mix enables accurate and uniform amplification of DNA and cDNA libraries while preserving library complexity and minimizing amplification bias.

At the core of this master mix is the advanced Platinum™ SuperFi™ DNA Polymerase, a high-performance proofreading polymerase with strong 3′→5′ exonuclease activity. This ensures extremely low error rates, delivering amplification accuracy comparable to PCR-free workflows. The proprietary reaction buffer included in the mix contains all essential PCR components, enabling efficient amplification across diverse templates, including GC-rich and structurally complex regions.

The master mix incorporates Platinum hot-start technology, which prevents polymerase activity at ambient temperatures. This allows flexible reaction setup at room temperature and improves specificity while reducing nonspecific amplification and primer-dimer formation. Additionally, the mix contains an inert blue tracking dye, providing visual confirmation during pipetting and minimizing setup errors.

Designed for high sensitivity and efficiency, the Collibri Library Amplification Master Mix enables reliable amplification even from low-input sequencing libraries. It is ideal for RNA-Seq, DNA sequencing, transcriptomics, and other Illumina-based NGS workflows requiring accurate and reproducible library enrichment.

Key Features

• High fidelity amplification: Platinum SuperFi DNA Polymerase ensures extremely low error rates
• Minimal amplification bias: Uniform amplification across GC-rich and AT-rich regions
• Hot-start PCR technology: Improves specificity and allows flexible reaction setup
• Ready-to-use master mix: Includes polymerase, buffer, and essential PCR components
• High amplification efficiency: Generates high yields from low input library material
• Visual pipetting aid: Blue tracking dye improves workflow accuracy
• Optimized for NGS workflows: Ideal for Illumina sequencing library preparation
• Automation compatible: Suitable for manual and automated high-throughput workflows

Applications

• Next-generation sequencing (NGS) library amplification
• RNA sequencing (RNA-Seq) library amplification
• DNA sequencing library preparation
• cDNA library amplification
• Transcriptome sequencing workflows
• Illumina library preparation workflows
• Genomics and transcriptomics research

Technical Specifications

Workflow Integration

RNA Extraction → Library Preparation → Cleanup → Library Amplification → Library Quantification → Illumina Sequencing

Advantages

• Ensures highly accurate amplification of sequencing libraries
• Preserves library complexity and sequence representation
• Minimizes amplification errors and bias
• Supports low-input and complex library amplification
• Essential for reliable Illumina NGS workflows

The Invitrogen™ Collibri™ Library Amplification Master Mix provides a reliable, high-fidelity solution for amplifying sequencing libraries in Illumina NGS workflows. Its advanced proofreading polymerase, optimized buffer chemistry, and hot-start technology ensure accurate, uniform, and efficient amplification, making it an essential reagent for transcriptomics, genomics, and next-generation sequencing applications.