Invitrogen™ 4X Bolt™ LDS Sample Buffer

Invitrogen™ Bolt™ LDS Sample Buffer (4X) is a high-performance sample loading buffer formulated for denaturing gel electrophoresis using Bolt™ Bis-Tris Plus gels. The buffer contains lithium dodecyl sulfate (LDS) at pH 8.5, which supports optimal activity of reducing agents and ensures efficient protein denaturation.

Designed for compatibility with MES and MOPS SDS running buffers, the buffer includes Coomassie G250 and Phenol Red tracking dyes instead of bromophenol blue. This ensures a sharper dye front and prevents small peptides from running off the gel, especially when using MES SDS buffer.

The buffer also features a highly viscous and concentrated formulation, with higher levels of LDS and glycerol than conventional SDS sample buffers, providing better loading consistency and gel compatibility.

Key Features and Benefits

• 4X concentrated format – dilute to 1X for sample preparation
• LDS-based buffer – optimized for denaturing proteins
• Coomassie G250 + Phenol Red dyes – improved visualization and separation
• Compatible with MES/MOPS SDS buffers and Bolt™ Bis-Tris Plus gels
• Maintains peptide resolution – prevents small peptides from running off the gel
• Ready-to-use – just add to protein samples and heat at 70 °C for 10 minutes

Recommended Use

• Dilute to 1X with protein sample
• Heat at 70 °C for 10 minutes (reduced or non-reduced format)
• Allow buffer to reach room temperature (25 °C) before use for proper viscosity

Applications

• SDS-PAGE sample preparation
• Denaturing electrophoresis of proteins
• Western blotting and protein detection workflows
• Compatible with Bolt™ Mini Gel Tank and other Invitrogen electrophoresis systems

Technical Specifications

Invitrogen™ 4X Bolt™ LDS Sample Buffer is engineered for high-efficiency protein sample preparation, providing superior performance in denaturing gel electrophoresis. With its optimized LDS-based formulation, high glycerol content, and tracking dyes that enhance resolution, it is the ideal sample buffer for SDS-PAGE using Bolt™ Bis-Tris Plus gels.