Invitrogen™ TRIzol™ Reagent

The TRIzol™ Reagent is a reliable, ready-to-use solution for the efficient isolation of high-quality RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate ensures simultaneous isolation of multiple biomolecules, making it a versatile choice for molecular biology applications.

Key Features

1. Multitarget Isolation:
   • Isolate RNA, DNA, and proteins from the same sample with sequential precipitation steps.
2. Wide Sample Compatibility:
   • Suitable for a variety of samples, including human, animal, plant, yeast, and bacterial cells and tissues.
3. Efficient RNA Recovery:
   • Total RNA is free of protein and DNA contamination, ensuring high purity for downstream applications.
4. Time-Efficient Protocol:
   • Complete the entire isolation process within 1 hour, facilitating simultaneous processing of multiple samples.
5. High Yield:
   • Recover RNA (≤73 μg), DNA (≤7 μg), and protein from small to large sample sizes, including tissues and cultured cells.
6. Optimized Formulation:
   • Protects RNA integrity by effectively inhibiting RNase activity during lysis and homogenization.

Specifications

Applications

1. RNA Applications:
   • RT-PCR, qPCR, microarray analysis, nuclease protection assays, Northern blotting, and RNA-seq.
2. DNA Applications:
   • Southern blotting, NGS, cDNA library construction, and hybridization studies.
3. Protein Applications:
   • Western blotting, 2D gel electrophoresis, immunoprecipitation, and enzymatic assays.

Advantages

Comparison with Competitor Products

Workflow Overview

1. Sample Homogenization:
   • Mix TRIzol™ Reagent with the sample (tissue, cells, etc.) to lyse cells and dissolve cellular components.
2. Phase Separation:
   • Add chloroform, mix, and centrifuge to separate the sample into three phases:
     • Aqueous Phase: RNA
     • Interphase: DNA
     • Organic Phase: Protein
3. Precipitation:
   • Precipitate RNA, DNA, and protein separately using isopropanol or ethanol.
4. Washing and Resuspension:
   • Wash precipitated molecules to remove impurities and resuspend them in an appropriate buffer.

Suggested Pairing

• Chloroform:
  • Use Invitrogen™ UltraPure™ Phenol for enhanced performance during phase separation.
• RNA Amplification:
  • Combine with Invitrogen™ SuperScript™ IV RT for robust RNA amplification.
• DNA Applications:
  • Use with Invitrogen™ Platinum™ Taq DNA Polymerase for reliable PCR results.

The TRIzol™ Reagent is an industry-standard solution trusted by researchers worldwide for its robust performance and versatility in nucleic acid and protein isolation, making it an invaluable tool for molecular biology workflows.