Invitrogen™ Qubit™ Protein Broad Range (BR) Assay Kits

Quantify protein quickly and accurately with the Qubit Protein BR Assay Kit, which quantifies 0.1 to 20 mg/mL of protein. The assay is compatible with many common contaminants, reducing reagents, salts, and nucleic acids. The Protein BR Assay Kit is also tolerant of detergents.

Qubit Protein BR Assay Kit

The Qubit Protein BR Assay Kit provides a quick, easy, and reliable method for determining the protein concentration of purified proteins, lysates, serum, plasma, or complex protein mixtures using a Qubit 4 Fluorometer. This protein kit can deliver accurate protein quantitation across a broad range (0.1–20 mg/mL), enabling most samples to be used neat (undiluted) and eliminating the guess work and dilution steps that accompany traditional protein quantitation methods. The assay is compatible with many common contaminants, such as detergents, reducing reagents (e.g., DTT, β-mercaptoethanol), salts, and nucleic acids.

Key features of the Qubit Protein BR Assay Kit include:

• Broad dynamic range—0.1 to 20 mg/mL, measure samples without dilution

• Two-point standard curve—eliminate time-consuming standard preparation

• Automatic concentration determination—receive protein concentration instantaneously; no need for offline calculations

• Compatible—accurate in the presence of reducing reagents, detergents, and other common buffer components

The Qubit Protein BR Assay Kit comes with two calibration standards, Protein BR Assay Buffer, and Protein BR Assay Reagent. Simply add 150–160 μL of Protein BR Assay Buffer to 10 or 20 μL of your sample, followed by 30 μL of Protein BR Assay Reagent. Incubate for 10 minutes at room temperature. Then insert the sample into a Qubit 4 instrument and read the concentration.

Notes

• The Qubit Protein BR Assay Kit is a fluorescence-based assay intended for use only with the Qubit 4 Fluorometer

• 500 μL thin-walled clear PCR tubes (Cat. No. Q32856) are required but not included in the Protein Assay and BR Assay Kits