Invitrogen™ Qubit™ Protein Assay Kits

Quantify protein quickly and accurately with the Qubit Protein Assay, which quantifies 12.5 μg/mL to 5 mg/mL of protein. This assay is compatible with many common contaminants, reducing reagents, salts, and nucleic acids.

Qubit Protein Assay Kit

The Qubit Protein Assay Kit is designed specifically for use with any Qubit fluorometer. While using between 1 and 20 μL of sample, this assay can quantify samples ranging from 12.5 μg/mL to 5 mg/mL with low protein-to-protein variation.

The Qubit Protein Assay Kit is highly selective for proteins and is also highly accurate in the presence of reducing reagents, but not in the presence of large amounts of detergent. Common contaminants, such as reducing reagents (e.g., DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, and DNA (but not detergents), are well tolerated in the assay. Slight protocol modifications are required for other contaminants.

The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration.

Which product should you choose for fluorometric protein quantitation?

• 1–20 samples: use this Qubit Protein Assay Kit with the Qubit Fluorometer

• 20–2000 samples: use the Quant-iT Protein Assay Kit with a microplate reader  

Notes

• The Qubit Protein Assay Kit can be used with Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers

• 500 μL thin-walled clear PCR tubes (Cat. No. Q32856) are required but not included in the Protein Assay and BR Assay Kits