Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Reagent, 50 pg to 2 μg

The Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Reagent is a highly sensitive fluorescent dye specifically designed for double-stranded DNA (dsDNA) quantification. It provides a broad dynamic range (50 pg to 2 μg) and is significantly more sensitive than UV absorbance methods, making it ideal for low-concentration DNA measurements.

The PicoGreen™ dye exhibits strong fluorescence enhancement upon binding to dsDNA, with excitation/emission maxima at 502/523 nm, allowing for precise quantification using fluorometers and fluorescence microplate readers.

📌 Two Packaging Options:

• P7581 – 1 x 1 mL Bottle (sufficient for ~200 assays)
• P11495 – 10 x 100 µL Tubes (small-volume format for reduced waste)

📌 Key Features:

• Highly specific for dsDNA – Minimal interference from ssDNA, RNA, or nucleotides.
• Rapid and easy workflow – Mix with sample, incubate for 5 minutes, and measure fluorescence.
• Compatible with multiple platforms – Works with fluorometers and microplate readers.

Key Features & Benefits

1. High Sensitivity & Specificity for dsDNA

✔ Detects dsDNA as low as 50 pg – Ideal for low-concentration samples.

✔ No interference from ssDNA & RNA – Ensures accurate quantification of dsDNA.

✔ Higher sensitivity than UV absorbance methods – Conserves precious DNA samples.

2. Simple & Fast Fluorescent DNA Quantitation

✔ One-step, mix-and-read protocol – Reduces pipetting steps and eliminates the need for enzymatic reactions.

✔ Short incubation time (5 minutes) – Provides quick and reliable DNA quantification.

✔ No DNA extraction required – Works directly with various DNA sources.

3. Broad Dynamic Range (50 pg to 2 μg)

✔ Linear fluorescence response over three orders of magnitude – Suitable for a wide range of DNA concentrations.

✔ Consistent quantification across different DNA sources – Works with genomic DNA, viral DNA, plasmid DNA, PCR amplicons, and microarray samples.

4. Flexible Compatibility

✔ Works with microplate readers & fluorometers – Excitation at 502 nm, Emission at 523 nm.

✔ Suitable for 96- and 384-well plate formats – Ideal for high-throughput screening.

✔ Stable & long shelf-life – Store at 2–8°C for long-term use.

Technical Specifications

Applications & Use Cases

1. PCR & qPCR Sample Quantification

🧬 Ensures precise template input for high-efficiency amplification.

🧬 Quantifies dsDNA before PCR or qPCR setup to optimize reaction conditions.

2. Genomic DNA Quantification

🧪 Accurately measures DNA for sequencing and cloning workflows.

🧪 Works with genomic DNA, viral DNA, and plasmid DNA from various sources.

3. Microarray Sample Preparation

🧫 Ensures uniform DNA concentrations for hybridization experiments.

🧫 Works with low-input DNA samples for high-throughput analysis.

4. Viral & Microbial DNA Quantification

🦠 Accurately quantifies viral genomes for diagnostic and research applications.

🦠 Measures low-copy DNA samples without PCR pre-amplification.

5. DNA Damage & Repair Assays

🧬 Assesses DNA degradation and repair efficiency in experimental studies.

🧬 Works in environmental, forensic, and biomedical research.

6. Enzyme Activity & DNA-Binding Protein Studies

🔬 Detects dsDNA binding proteins and their interactions.

🔬 Monitors enzymatic degradation of DNA by nucleases.

Workflow & Protocol

1️⃣ Prepare the Working Solution:

• Dilute PicoGreen™ reagent 1:200 in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.5–8.0).

2️⃣ Add DNA Sample & Incubate:

• Mix equal volumes of sample and PicoGreen™ working solution.
• Incubate for 5 minutes at room temperature in the dark.

3️⃣ Measure Fluorescence:

• Detect fluorescence at Excitation 502 nm / Emission 523 nm using a fluorometer or microplate reader.

Ordering Information

📦 Shipping Conditions: Room Temperature

🧊 Storage Conditions: 2–8°C (Light-Sensitive)

Why Choose Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Reagent?

✅ High Sensitivity – Detects dsDNA at levels as low as 50 pg.

✅ Simple & Rapid Protocol – 5-minute incubation, one-step quantification.

✅ Compatible with Fluorometers & Microplate Readers – Works in 96- and 384-well formats.

✅ dsDNA-Specific Assay – Minimal interference from RNA and free nucleotides.

✅ Optimized for PCR, NGS, and Microarray Applications – Ensures consistent and accurate DNA measurements.

The Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Reagent is a powerful, highly sensitive DNA quantitation solution, perfect for low-concentration DNA samples, PCR preparation, sequencing, and molecular biology research. Its simple workflow, broad dynamic range, and compatibility with fluorescence detection platforms make it an essential tool for genomic and biomedical applications.

🔍 Ideal for: Molecular biologists, genomics researchers, forensic scientists, and clinical diagnostics professionals.