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Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
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CAS Number :
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Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase is a highly efficient and robust PCR enzyme designed for fast, reliable, and specific DNA amplification. Its innovative buffer system, engineered Taq polymerase, and hot-start technology allow for universal primer annealing and streamlined PCR workflows, even with challenging samples.
Key Features
- Innovative Buffer:
- Enables universal primer annealing temperature (60°C) for most primer pairs.
- Isostabilizing molecules enhance primer-template duplex stability, eliminating the need for primer-specific optimization.
- Engineered Taq Polymerase:
- Delivers faster cycling and increased resistance to inhibitors.
- Provides reliable amplification of DNA from complex templates, including GC-rich regions.
- Platinum Hot-Start Technology:
- Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate at 94°C.
- Enhances specificity, sensitivity, and yields while enabling room temperature reaction setup.
- Versatile Applications:
- Suitable for a wide range of DNA templates, including genomic, plasmid, and viral DNA.
- Works well for high-throughput PCR, genotyping, RT-PCR, and amplification from low-purity samples.
Specifications
| Parameter | Details |
|---|---|
| Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
| Buffer | 5X Platinum II PCR Buffer |
| Optional Additive | Platinum GC Enhancer |
| Hot Start Mechanism | Platinum Taq Antibodies |
| Reaction Speed | Fast or Standard |
| Annealing Temperature | Universal 60°C |
| GC-Rich PCR Performance | High |
| Overhang | 3'-A |
| Size (Final Product) | ≤5 kb |
| Fidelity | 1X (compared to standard Taq) |
| Exonuclease Activity | 5' - 3' |
| Storage Temperature | -20°C (non-frost-free freezer) |
| Shipping Condition | Dry Ice |
| Product Type | Hot Start DNA Polymerase |
Product Formats
| Catalog Number | No. of Reactions | Description |
|---|---|---|
| 14966100 | 4 × 2500 | Bulk size for high-throughput labs |
| 14966001 | 100 | Small-scale experiments |
| 14966005 | 500 | Medium-scale applications |
| 14966025 | 2500 | Large-scale workflows |
Applications
- Routine PCR
- Genotyping
- RT-PCR
- High-throughput PCR
- Amplification of GC-rich DNA
- DNA amplification from complex templates
- Colony PCR
Advantages
- Streamlined Workflow:
- Universal annealing temperature simplifies setup across different assays.
- Optional Platinum GC Enhancer improves yields for GC-rich templates.
- Faster Cycling:
- Engineered polymerase enables faster reaction times compared to conventional Taq.
- Convenient Formats:
- Master mix options available for reduced pipetting and enhanced workflow efficiency.
- Direct gel loading with Platinum Green PCR Master Mix (2X).
- Inhibitor Resistance:
- Amplifies DNA even from impure samples or inhibitor-rich extracts.
- Reliable Performance:
- High specificity and sensitivity ensure accurate and reproducible results.
Master Mix Options
| Master Mix | Features |
|---|---|
| Platinum II Hot-Start PCR Master Mix (2X) | Pre-mixed with dNTPs and buffer for faster setup. |
| Platinum II Hot-Start Green PCR Master Mix (2X) | Includes tracking dyes and density reagent for direct gel loading. |
Contents
Each pack includes:
- Platinum II Taq Hot-Start DNA Polymerase
- 5X Platinum II PCR Buffer
- Platinum GC Enhancer (optional for GC-rich templates)
Storage and Handling
- Storage Temperature: Store at -20°C in a non-frost-free freezer.
- Shipping Conditions: Shipped on dry ice to maintain enzyme stability.
Comparison: Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase vs. OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
| Feature | Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase | OneTaq® Quick-Load® 2X Master Mix with Standard Buffer |
|---|---|---|
| Type | Hot-start DNA polymerase (separate components) | Ready-to-use 2X master mix with buffer and dNTPs |
| Format | Separate components (enzyme, buffer, GC enhancer) | Pre-mixed master mix with tracking dyes (Quick-Load format) |
| Hot Start Mechanism | Antibody-based Platinum technology | Chemically modified hot-start DNA polymerase |
| Reaction Setup | Room temperature setup | Room temperature setup |
| Universal Primer Annealing | Yes, with universal annealing temperature (60°C) | No, annealing temperature varies based on primers |
| Amplicon Size Range | Up to 5 kb | Up to 10 kb for genomic DNA, up to 40 kb for lambda DNA |
| GC-Rich Template Performance | High, with optional GC enhancer | High, with robust amplification for GC-rich templates |
| Direct Gel Loading | Yes, with Platinum II Hot-Start Green PCR Master Mix | Yes, includes density reagents and tracking dyes in Quick-Load format |
| Applications | - Routine PCR | - Routine PCR |
| - Genotyping | - Colony PCR | |
| - High-throughput PCR | - Long-template PCR | |
| - Amplification of GC-rich templates | - GC-rich template amplification | |
| - RT-PCR | - DNA sequencing preparation | |
| Reaction Speed | Fast or standard | Standard |
| Buffer | 5X Platinum II PCR Buffer | Standard buffer in master mix |
| Polymerase | Platinum II Taq Hot-Start DNA Polymerase | OneTaq DNA Polymerase |
| Overhang | 3'-A | 3'-A |
| Fidelity (vs. Taq) | 1X | 1X |
| Concentration | 5X buffer supplied separately | 2X master mix |
| Certifications | Research use only | ISO-certified for quality |
| Storage Temperature | -20°C (non-frost-free freezer) | -20°C |
| Shipping Condition | Dry ice | Dry ice |
| Best Use Cases | - Universal annealing for multiple assays | - Long-template amplification |
| - High-throughput and complex PCR | - Routine PCR requiring minimal optimization | |
| Packaging Options | - 100, 500, 2500, 4 × 2500 reactions | - 50, 200, 1000 reactions |
Key Differences
- Reaction Setup and Buffer:
- Platinum™ II: Includes a 5X buffer and requires separate addition of enzyme, buffer, and optional GC enhancer.
- OneTaq® Quick-Load®: Pre-mixed 2X format with fewer setup steps, ideal for simplicity.
- Universal Annealing:
- Platinum™ II: Features a universal annealing temperature of 60°C for most primers, eliminating optimization.
- OneTaq®: Requires primer-specific annealing temperature optimization.
- Amplicon Size:
- Platinum™ II: Best for fragments up to 5 kb.
- OneTaq®: Suitable for longer templates (up to 10 kb for genomic DNA, 40 kb for lambda DNA).
- GC-Rich Templates:
- Both perform well with GC-rich templates, though Platinum™ II provides an optional enhancer for added robustness.
- Direct Gel Loading:
- Both offer direct loading options, but OneTaq® Quick-Load® includes this feature in its standard product.
Choose Platinum™ II Taq Hot-Start for advanced workflows requiring universal primer annealing and fast cycling. Select OneTaq® Quick-Load® for simplicity and long-template amplification.
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase is a highly versatile and efficient PCR reagent, designed to enhance specificity, sensitivity, and speed. Its universal annealing temperature and inhibitor resistance make it a reliable choice for a wide range of DNA amplification applications, from routine PCR to challenging GC-rich templates.
Application Notes
Application Note: Multiplex PCR using Platinum II Taq Hot-Start DNA Polymerase
Application Note: Direct PCR from blood using Platinum II Taq Hot-Start DNA Polymerase
Application Note: Bacterial DNA detection using Platinum II Taq Hot-Start DNA Polymerase
Application Note: Genotyping from mouse tail using Platinum II Taq Hot-Start DNA Polymerase
- Reactions: 100 rxn 500 rxn 2500 rxn 4 x 2500 rxn