Invitrogen™ One Shot™ LB Agar Plates

One Shot LB Agar Plates are pre-poured Luria-Bertani (LB) agar plates used for the cultivation and maintenance of Escherichia coli (E. coli) strains. These plates are ideal for molecular biology studies, including cloning and bacterial transformation experiments. They are available with or without antibiotics to suit different experimental needs, making them versatile for a variety of cloning and selection applications.

The inclusion of autoclaved glass beads facilitates easier plate inoculation without the need for additional loops or spreaders, ensuring a clean and efficient workflow.

Key Features & Benefits:

1. Pre-Poured and Ready-to-Use:
   • These plates are pre-poured, eliminating the need for preparation and reducing the risk of contamination.
   • They come conveniently packaged in two sleeves of 10 plates, ready for immediate use.
2. Enhanced Cloning Applications:
   • The LB agar medium is based on the Jeffrey H. Miller formulation, which includes 10 g/L casein peptone, 5 g/L yeast extract, 10 g/L NaCl, and 15 g/L agar, providing a nutrient-rich environment for E. coli growth and easy isolation of single colonies.
   • The formulation also contains twice as much sodium chloride compared to the Lennox formulation, ensuring optimal osmotic balance for E. coli growth.
3. Antibiotic Selection:
   • Available with a variety of antibiotics, including 100 μg/mL Ampicillin or 50 μg/mL Kanamycin, or without antibiotics for general growth.
   • The antibiotic selection ensures only transformed cells with resistance can grow, making it ideal for selecting recombinant clones.
4. Autoclaved Glass Plating Beads:
   • Each plate includes autoclaved glass plating beads, simplifying the cell spreading process without the need for additional tools like loops or spreaders.
5. Long Shelf Life:
   • Long shelf life of up to 3.5 months without antibiotics and 3 months with antibiotics, ensuring that you have reliable plates for long-term storage and future use.
6. Supports a Variety of Applications:
   • Perfect for cloning, streaking, and isolating bacterial cells.
   • Can also be used for bacteriophage plaque assays by growing a lawn of bacteria on the plate.

Applications:

• Cloning: Use for transforming E. coli cells with plasmid vectors and selecting for recombinant clones.
• Bacterial Isolation: Ideal for isolating individual bacterial colonies from mixed cultures.
• Bacteriophage Plaque Assay: Suitable for performing bacteriophage plaque assays by growing bacteria on the plates and observing phage-induced plaques.
• General Bacterial Growth: Ideal for growing E. coli and coliforms on selective and non-selective media.

Specifications:

Invitrogen™ One Shot™ LB Agar Plates provide a convenient, reliable, and efficient solution for bacterial cloning, transformation, and isolation. With their pre-poured format, antibiotic selection options, and autoclaved glass beads, they support a wide range of applications in molecular biology. Their extended shelf life and compatibility with high-efficiency cloning protocols make them an excellent choice for research labs involved in genetic manipulation, gene expression studies, and bacterial characterization.