Invitrogen™ NaCl (5 M), RNase-free

The Invitrogen™ Ambion™ 5 M Sodium Chloride (NaCl) Solution is a high-purity, RNase-free reagent designed for molecular biology applications. Manufactured under stringent nuclease-free conditions, this ready-to-use solution is certified free of RNase, DNase, and protease activity, making it ideal for RNA research, nucleic acid purification, and enzymatic reactions.

Key Features & Benefits

1. RNase-Free & High-Purity Molecular Biology Grade

• Certified RNase-, DNase-, and protease-free, ensuring RNA stability and integrity.
• Manufactured in specialized nuclease-free facilities, reducing contamination risk.
• Highly sensitive quality control assays are conducted at multiple production stages.

2. Ready-to-Use, Pre-Diluted 5 M Solution

• Eliminates the need for weighing and dissolving NaCl, saving preparation time.
• Ensures accurate concentration, avoiding errors in buffer preparation.
• Economical and convenient for routine laboratory use.

3. Essential Reagent for Molecular Biology & Biochemistry

• Used in RNA isolation, hybridization, and nucleic acid purification.
• Maintains ionic strength in buffers for PCR, electrophoresis, and enzymatic assays.
• Suitable for protein extraction, immunoprecipitation, and Western blot washing buffers.

Specifications

Applications

1. Molecular Biology & RNA Applications

• RNA stability and purification – Ensures an RNase-free environment.
• Used in nucleic acid extraction buffers for DNA/RNA separation.
• Commonly included in Northern and Southern blot hybridization buffers.

2. PCR, RT-PCR, and Enzyme Reactions

• Maintains optimal ionic strength for DNA polymerase activity.
• Used in PCR, qPCR, and RT-PCR buffer preparations.
• Enhances enzyme performance in restriction digestion and ligation reactions.

3. Protein Purification & Biochemical Assays

• Used in protein extraction and precipitation protocols.
• Component of Western blot wash buffers and cell lysis buffers.
• Adjusts osmotic balance in cell culture media and buffer solutions.

Pack Sizes & Catalog Numbers

Storage & Handling

• Store at room temperature for long-term stability.
• Use sterile techniques when handling to maintain RNase-free conditions.
• Keep container tightly closed to prevent contamination.

The Invitrogen™ Ambion™ 5 M Sodium Chloride (NaCl) Solution is a high-purity, RNase-free reagent optimized for molecular biology, RNA research, and biochemical applications. Its ready-to-use format eliminates preparation errors and ensures consistent results in nucleic acid and protein workflows.