Invitrogen™ IL-2 Human ELISA Kit, 96 Tests

The Invitrogen™ Human IL-2 ELISA Kit (BMS221-2) is a high-sensitivity sandwich ELISA assay designed for quantifying interleukin-2 (IL-2) in human serum, plasma, and cell culture supernatants. With an analytical sensitivity of 9.1 pg/mL and a broad dynamic range (18.8–1,200 pg/mL), this kit ensures accurate and reproducible IL-2 detection, making it ideal for immunology, inflammation, and cytokine research.

Featuring a pre-coated 96-well plate and ready-to-use reagents, this ELISA kit enables efficient workflow execution, with a total time-to-result of 3 hours 30 minutes. Each lot undergoes rigorous validation for sensitivity, specificity, and reproducibility, ensuring consistent high-quality results.

Key Features & Benefits

1. High Sensitivity & Wide Dynamic Range

• Analytical sensitivity: 9.1 pg/mL, ensuring detection of low-abundance IL-2.
• Assay range: 18.8–1,200 pg/mL, suitable for various biological sample types.

2. Fast & Efficient Workflow

• Total assay time: 3 hours 30 minutes, with only 1 hour 20 minutes hands-on time.
• Pre-coated microplate and ready-to-use reagents simplify workflow and increase efficiency.

3. Reliable & Reproducible Performance

• Low interassay CV (5%) and intraassay CV (7%), ensuring consistent and precise results.
• Validated for human serum, plasma, and supernatants.

4. Robust & High-Specificity Sandwich ELISA Format

• Capture antibody pre-coated onto microplate wells ensures high binding affinity for IL-2.
• Detection antibody conjugated to biotin, with streptavidin-HRP for signal amplification.
• Colorimetric detection via HRP-TMB substrate, optimized for microplate readers.

5. Comprehensive Kit Components for Easy Setup

• Includes all necessary reagents for a streamlined workflow:
  • Pre-coated 96-well microplate
  • Biotinylated detection antibody & Streptavidin-HRP
  • IL-2 standard & Sample diluent
  • Assay buffer concentrate & Wash buffer
  • Chromogen & Stop solution
  • Adhesive plate covers

Specifications

Available Variants & Ordering Information

Use Cases & Applications

1. Immunology & Inflammation Research

• Quantifies IL-2 in immune system activation and regulatory responses.
• Ideal for studying T-cell proliferation and differentiation.
• Supports research in autoimmune diseases, such as multiple sclerosis and rheumatoid arthritis.

2. Infectious Disease & Vaccine Research

• Measures IL-2 secretion in response to viral and bacterial infections.
• Helps evaluate vaccine-induced immune responses and immunotherapy efficacy.

3. Cancer & Immunotherapy Studies

• Investigates IL-2’s role in tumor immunity and cancer immunotherapy.
• Supports studies on T-cell activation and cytokine modulation in the tumor microenvironment.

4. Cytokine Profiling & Biomarker Discovery

• Enables multi-cytokine analysis in disease biomarker research.
• Facilitates cytokine profiling in cell culture supernatants for functional immunology studies.

Target Information: IL-2 (Interleukin-2)

Interleukin-2 (IL-2) is a key cytokine involved in T-cell proliferation, differentiation, and immune regulation. It is produced primarily by activated CD4+ and CD8+ T cells and plays a critical role in immune responses, inflammatory processes, and tumor immunity. IL-2 binds to the IL-2 receptor (IL-2R) and activates the JAK-STAT signaling pathway, promoting T-cell growth and differentiation. IL-2 dysregulation has been associated with autoimmune diseases, transplant rejection, and cancer.

Aliases:

• IL2, Interleukin-2, T-cell growth factor (TCGF), Aldesleukin

UniProt ID (Human): P60568

The Invitrogen™ Human IL-2 ELISA Kit (BMS221-2) is a high-sensitivity, validated ELISA assay designed for quantitative IL-2 measurement in human samples. With a broad dynamic range (18.8–1,200 pg/mL), minimal sample volume requirement (50 µL), and validated high-performance detection, this kit is an essential tool for immunology, cytokine research, and immunotherapy studies.