Invitrogen™ eBioscience™ Integrin beta 7 Monoclonal Antibody (FIB504)

The Invitrogen™ Integrin beta 7 Monoclonal Antibody (FIB504) is a high-quality, unconjugated monoclonal antibody designed for detecting Integrin beta 7, a 130 kDa glycoprotein expressed in human and mouse samples. Integrin beta 7 forms heterodimers with alpha 4 (CD49d) or alpha E (CD103), mediating leukocyte adhesion and migration. This antibody is validated for flow cytometry and functional assays, providing reliable performance for immune cell research, inflammation studies, and cell adhesion mechanisms.

Key Features:

1. Target and Specificity:
   • Recognizes Integrin beta 7, which is involved in immune cell adhesion and transmigration during inflammatory responses.
   • Expressed on intraepithelial lymphocytes, mucosal T cells, and other immune cell subsets.
2. Clone:
   • Clone: FIB504, ensuring high specificity and sensitivity.
3. Validated Applications:
   • Optimized for flow cytometry and functional assays, enabling robust analysis of Integrin beta 7 expression and function.
4. High Purity:
   • Greater than 90% purity, as determined by SDS-PAGE.
   • Less than 10% aggregation, as confirmed by HPLC.
5. Unconjugated Format:
   • Allows flexibility in pairing with secondary antibodies or labeling reagents for custom experimental needs.
6. Ready-to-Use:
   • Provided in liquid form, pre-titrated for ease of use, with a concentration of 0.5 mg/mL.

Specifications:

Applications:

1. Flow Cytometry:
   • Detects Integrin beta 7 expression on lymphocytes and other immune cell populations.
   • Suitable for staining human and mouse peripheral blood cells and intraepithelial lymphocytes.
2. Functional Assays:
   • Investigate the role of Integrin beta 7 in leukocyte adhesion and migration.
3. Immunophenotyping:
   • Analyze lymphocyte subsets expressing Integrin beta 7 in human and mouse samples.
4. Adhesion Molecule Research:
   • Study interactions between Integrin beta 7 and its ligands, including VCAM-1, MadCAM-1, and E-cadherin.
5. Inflammation and Immune Response Studies:
   • Examine leukocyte transmigration to inflamed tissues mediated by Integrin beta 7.

Key Benefits:

1. High Specificity:
   • The FIB504 clone ensures reliable detection of Integrin beta 7 with minimal background noise.
2. Reproducible Results:
   • Validated for flow cytometry and functional assays, ensuring consistent performance across experiments.
3. Flexibility:
   • Unconjugated format allows pairing with secondary antibodies or custom labeling for various experimental designs.
4. Quality Assurance:
   • High purity and low aggregation ensure robust and reproducible experimental outcomes.

Usage Recommendations:

1. Sample Preparation:
   • Stain 10⁵–10⁸ cells per test in a final volume of 100 µL using ≤0.125 µg of antibody.
2. Flow Cytometry:
   • Use with a secondary antibody (e.g., PE or FITC-conjugated) for signal detection.
3. Functional Assays:
   • Determine optimal concentration based on specific assay requirements.
4. Storage and Handling:
   • Store at 4°C. Avoid freezing to preserve antibody integrity.

Target Information:

• Integrin beta 7:
  • A key adhesion molecule that associates with integrin alpha 4 (CD49d) to form alpha 4 beta 7 (LPAM-1) or with integrin alpha E (CD103) to form alpha E beta 7 (HML-1).
  • Plays a critical role in mucosal immunity and immune cell migration to inflamed tissues.
  • Binds to VCAM-1, MadCAM-1, and E-cadherin, mediating immune cell adhesion and transmigration.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.