Invitrogen™ eBioscience™ IL-17A Monoclonal Antibody (eBio64DEC17), APC

The Invitrogen™ IL-17A Monoclonal Antibody (eBio64DEC17), conjugated to APC (Allophycocyanin), is a highly specific antibody designed for detecting human IL-17A in intracellular staining followed by flow cytometric analysis. IL-17A is a cytokine secreted by Th17 cells and plays a critical role in inflammatory responses and immune regulation. This antibody is validated for intracellular staining and offers bright fluorescence for reliable detection.

Key Features:

1. Target and Specificity:
   • Recognizes human IL-17A, a 32 kDa cytokine involved in promoting inflammation and immune responses.
   • Neutralizing antibody with high specificity for IL-17A.
2. Clone and Conjugate:
   • Clone: eBio64DEC17, ensuring reliable and reproducible performance.
   • Conjugate: APC, providing bright and stable fluorescence with excitation at 651 nm and emission at 660 nm.
3. Validated Applications:
   • Optimized for intracellular staining and flow cytometry to study IL-17A expression in polarized Th17 cells and other immune cells.
4. Bright and Stable Fluorescence:
   • APC fluorochrome offers high signal intensity for clear separation of IL-17A-positive and negative populations.
5. Ready-to-Use:
   • Pre-titrated for ease of use, with 5 µL (0.06 µg) sufficient for each test.

Specifications:

Applications:

1. Intracellular Staining:
   • Detect IL-17A expression in CD4+ T cells, including Th17-polarized cells.
   • Analyze cytokine production in immune cells under stimulated conditions.
2. Flow Cytometry:
   • Ideal for use in multicolor panels to identify IL-17A-producing cells in human samples.
3. Functional Assays:
   • Investigate the role of IL-17A in inflammatory responses, autoimmunity, and disease models.
4. Th17 Research:
   • Study IL-23-dependent Th17 differentiation pathways and their role in inflammatory diseases.
5. Inflammation Studies:
   • Examine IL-17A involvement in conditions such as rheumatoid arthritis, asthma, multiple sclerosis, and psoriasis.

Key Benefits:

1. High Specificity and Sensitivity:
   • Detects IL-17A with minimal cross-reactivity or background.
2. Bright Fluorescence:
   • APC provides a strong and stable signal for clear identification of IL-17A-positive cells.
3. Reproducible Results:
   • Pre-titrated antibody ensures consistency and reliability across experiments.
4. Flexibility:
   • Compatible with various fixation and permeabilization protocols for intracellular staining.
5. Validated for Research:
   • Optimized for studying IL-17A production in human immune cells using flow cytometry.

Usage Recommendations:

1. Sample Preparation:
   • Polarize human peripheral blood CD4+ T cells under Th17 conditions.
   • Stimulate cells using appropriate reagents (e.g., Cell Stimulation Cocktail).
2. Staining Protocol:
   • Use the Intracellular Fixation & Permeabilization Buffer Set for effective staining.
   • Add 5 µL (0.06 µg) of antibody per 100 µL of cell suspension.
3. Flow Cytometry:
   • Analyze samples using a flow cytometer equipped with a red laser (633–647 nm).
4. Fixation:
   • Fix samples in IC Fixation Buffer to preserve fluorescence for up to 3 days.

Storage and Handling:

• Store at 4°C in the dark to protect the APC fluorochrome.
• Do not freeze, as freezing may degrade the antibody or fluorochrome.

Target Information:

• IL-17A:
  • A pro-inflammatory cytokine secreted by Th17 cells.
  • Plays a key role in autoimmune diseases, inflammation, and immune responses against fungal and bacterial infections.
  • Stimulates fibroblasts, epithelial cells, and endothelial cells to produce cytokines such as IL-6, IL-8, and G-CSF.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.