Invitrogen™ eBioscience™ IFN gamma Monoclonal Antibody (MD-1), Functional Grade

The Invitrogen™ IFN gamma Monoclonal Antibody (MD-1) is a functional-grade antibody designed for detecting and neutralizing human IFN gamma. IFN gamma (Interferon gamma) is a key cytokine involved in immune responses, macrophage activation, and cytokine regulation. This antibody is validated for flow cytometry, neutralization assays, ELISA, and functional assays, making it an essential tool for studying Th1-mediated immune responses, cytokine activity, and signaling pathways.

Key Features:

1. Target and Specificity:
   • Recognizes human IFN gamma, a Th1 cytokine produced by activated T cells, NK cells, and B cells.
   • High specificity ensures accurate detection and functional neutralization in research applications.
2. Clone and Grade:
   • Clone: MD-1, a trusted clone known for reliable performance.
   • Functional Grade: Free of preservatives, making it suitable for neutralization assays and other in vitro applications.
3. Validated Applications:
   • Designed for flow cytometry, neutralization, ELISA, and functional assays, offering versatility in immune research.
4. High Purity and Low Endotoxin:
   • Greater than 90% purity by SDS-PAGE and endotoxin levels <0.001 ng/µg (LAL assay).
5. Ready-to-Use:
   • Supplied in liquid form at a concentration of 1 mg/mL.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect intracellular and surface-expressed IFN gamma in T cells, NK cells, and other immune cell populations.
2. Neutralization:
   • Potently neutralizes IFN gamma biological activity in in vitro and functional assays.
3. ELISA:
   • Serves as a capture antibody for detecting IFN gamma in sandwich ELISA assays.
4. Functional Assays:
   • Investigate IFN gamma-mediated signaling pathways and immune activation.
5. Th1 Research:
   • Study IFN gamma's role in Th1-mediated immunity and macrophage activation.
6. Cytokine Regulation:
   • Examine IFN gamma's synergistic effects with other cytokines in immune response modulation.

Key Benefits:

1. High Specificity and Sensitivity:
   • Detects and neutralizes human IFN gamma with minimal cross-reactivity or background.
2. Versatility:
   • Validated for multiple applications, including flow cytometry, ELISA, and neutralization assays.
3. High Purity and Low Endotoxin:
   • Ensures reproducibility and reliability for sensitive applications like neutralization assays.
4. Preservative-Free:
   • Functional grade formulation is optimized for in vitro neutralization and functional studies.
5. Reproducible Results:
   • Validated and pre-tested for consistent performance across a range of applications.

Usage Recommendations:

1. Neutralization:
   • Use 30 µg/mL of the antibody to neutralize 50% of the activity of 1 ng/mL IFN gamma in vitro.
2. Flow Cytometry:
   • Use in intracellular staining protocols for detecting IFN gamma production in activated T cells and NK cells.
3. ELISA:
   • Recommended as a capture antibody at concentrations of 1-4 µg/mL in sandwich ELISA assays.
4. Functional Assays:
   • Employ for studying IFN gamma’s antiviral, tumor-antiproliferative, and immunomodulatory activities.
5. Sample Preparation:
   • Ensure a sterile environment to maintain the integrity of the antibody in functional studies.

Storage and Handling:

• Store at 4°C in a sterile environment.
• Do not freeze, as freezing may compromise the antibody's activity.

Target Information:

• IFN gamma:
  • A dimeric glycoprotein cytokine (~45 kDa) secreted by T cells, NK cells, and B cells.
  • Regulates immune responses, activates macrophages, and enhances MHC class I and II expression.
  • Involved in cytokine signaling through the JAK-STAT pathway.
  • Plays a key role in antiviral and tumor-suppressive immune responses.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.