Invitrogen™ eBioscience™ CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), PE-Cyanine7

The Invitrogen™ CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), conjugated to PE-Cyanine7, is a high-performance reagent designed for detecting CD49d (Integrin alpha 4) in mouse samples. CD49d is a 150 kDa integrin alpha subunit that associates with beta1 (forming VLA-4) or beta7 to mediate cell adhesion via interaction with fibronectin, VCAM-1 (CD106), and MadCAM-1. This antibody is ideal for flow cytometry and provides a bright, photostable signal for accurate and reproducible data.

Key Features:

1. Target and Specificity:
   • Recognizes mouse CD49d, an integrin alpha4 subunit involved in cellular adhesion and migration.
   • Expressed on thymocytes, peripheral lymphocytes, monocytes, and eosinophils.
2. Clone and Conjugate:
   • Clone: R1-2, ensuring high specificity and sensitivity.
   • Conjugate: PE-Cyanine7, offering bright fluorescence with excitation at 488-561 nm and emission at 775 nm.
3. Validated Applications:
   • Designed and optimized for flow cytometry to analyze CD49d expression in mouse splenocytes and other immune cell populations.
4. Bright and Stable Signal:
   • PE-Cyanine7 provides robust fluorescence for clear separation of positive and negative populations in flow cytometry.
5. Pre-Titrated and Ready-to-Use:
   • Each test requires ≤0.5 µg of antibody, with a liquid formulation ready for immediate use.

Specifications:

Applications:

1. Flow Cytometry:
   • Detects CD49d (Integrin alpha 4) expression on thymocytes, lymphocytes, monocytes, and eosinophils.
   • Useful for analyzing adhesion molecule expression and immune responses.
2. Immunophenotyping:
   • Ideal for studying integrin-mediated adhesion and migration in mouse immune cell subsets.
3. Adhesion Molecule Research:
   • Investigate CD49d interactions with fibronectin, VCAM-1, and MadCAM-1.
4. Stem Cell and Tissue Studies:
   • Examine CD49d-mediated lymphocyte homing and migration to mucosal tissues.
5. Cancer and Infectious Disease Research:
   • Study the role of integrin alpha4beta1 and alpha4beta7 in tumor metastasis and immune responses in infectious diseases.

Key Benefits:

1. High Specificity and Sensitivity:
   • The R1-2 clone offers reliable detection of mouse CD49d with minimal background noise.
2. Bright Fluorescence:
   • PE-Cyanine7 provides a high-intensity, photostable signal for multicolor flow cytometry.
3. Ease of Use:
   • Pre-titrated and ready-to-use formulation simplifies experimental workflows.
4. Reproducible Results:
   • Validated for flow cytometry, ensuring consistent performance in mouse immune cell studies.
5. Versatile Research Tool:
   • Suitable for studies in immunology, cancer, and infectious diseases.

Usage Recommendations:

1. Sample Preparation:
   • Stain 10⁵–10⁸ cells per test in a final volume of 100 µL using ≤0.5 µg of antibody.
2. Flow Cytometry:
   • Analyze using a flow cytometer equipped with a blue, green, or yellow-green laser.
3. Fixation:
   • Use appropriate fixation buffers (e.g., IC Fixation Buffer or 1-step Fix/Lyse Solution) to preserve fluorescence after staining.

Storage and Handling:

• Store at 4°C in the dark to protect the PE-Cyanine7 fluorochrome.
• Do not freeze, as freezing can degrade the antibody and conjugate.

Target Information:

• CD49d (Integrin alpha 4):
  • A key adhesion molecule that associates with beta1 or beta7 integrins to mediate immune cell trafficking and tissue homing.
  • Interacts with ligands such as fibronectin, VCAM-1, and MadCAM-1.
  • Plays a critical role in mucosal immunity and immune cell migration.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.