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Invitrogen™ CD4 Monoclonal Antibody (GK1.5), Alexa Fluor™ 488, eBioscience™
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Specifications:
| Application | immunocytochemistry, Flow Cytometry, Immunohistochemistry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Rat Anti-Mouse CD4 | Fluorescent Antibody for Flow Cytometry and Immunofluorescence Applications
The Invitrogen™ CD4 Monoclonal Antibody (Clone GK1.5) conjugated to Alexa Fluor™ 488 is a high-affinity antibody specific to mouse CD4 (55 kDa), a co-receptor expressed on thymocytes, helper T cells, and dendritic cells. This antibody is widely validated and cited across numerous studies, with over 95 published figures and 200+ citations.
Engineered for flow cytometry, immunofluorescence, and other fluorescence-based assays, this antibody enables bright and reliable staining for phenotyping and functional studies of murine immune cell subsets.
Key Features and Benefits
- Clone GK1.5: Highly specific monoclonal antibody targeting murine CD4.
- Fluorescent Conjugate: Conjugated to Alexa Fluor™ 488 (Excitation: 499 nm, Emission: 520 nm) for compatibility with blue lasers.
- Validated Performance: Supported by >95 published figures across flow cytometry, IHC, ICC/IF, and functional assays.
- High Purity and Stability: Affinity-purified; supplied in PBS with 0.09% sodium azide; filtration grade (0.2 µm); DO NOT FREEZE.
Applications
- Flow Cytometry (tested): ≤ 0.25 µg/test (100 µL final volume)
- Immunocytochemistry (ICC/IF)
- Immunohistochemistry (Frozen & PFA-fixed)
- Functional Assays
Optimal staining requires titration for the specific assay and sample type.
Technical Specifications
| Property | Details |
|---|---|
| Clone | GK1.5 |
| Host / Isotype | Rat / IgG2b, kappa |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Species Reactivity | Mouse |
| Published Reactivity | Mouse, Human, Fish |
| Tested Applications | Flow Cytometry |
| Other Validated Applications | IHC, ICC/IF, Functional Assays |
| Concentration | 0.5 mg/mL |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 with 0.09% sodium azide |
| Storage Conditions | 4°C, store in the dark, do not freeze |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| Isotype Control Recommended | Rat IgG2b kappa Isotype Control, Alexa Fluor™ 488 (eB149/10H5) |
| RRID | AB_469893 |
Available Pack Sizes
| Catalog Number | Quantity | Format |
|---|---|---|
| 53-0041-80 | 25 µg | Liquid |
| 53-0041-82 | 100 µg | Liquid |
Target Information – CD4
CD4 is a glycoprotein expressed on the surface of T helper cells, as well as some monocytes, macrophages, and dendritic cells. It plays a critical role in T cell activation, antigen presentation, and HIV entry. In mice, CD4 expression is tightly regulated and can be detected in thymus, spleen, lymph nodes, and peripheral immune tissues.
Binding of the GK1.5 clone to CD4 is known to block interaction with clone RM4-5, a feature important for experimental design in multiplex staining panels.
Invitrogen™ CD4 Monoclonal Antibody (GK1.5), Alexa Fluor™ 488 is a trusted, publication-validated reagent ideal for the fluorescent identification and analysis of mouse CD4+ T cells. With strong specificity, bright fluorescence, and validated performance in flow cytometry and imaging applications, this antibody is a critical tool for immunology and infectious disease research.
- Pack Size: 25 µg 100 µg