Sigma-Aldrich Ionomycin calcium salt, 1mL

Ionomycin calcium salt is a potent calcium ionophore derived from Streptomyces conglobatus, available here as a convenient ready-made 1 mM solution in DMSO. It is designed to facilitate the transport of Ca²⁺ ions across biological membranes, allowing researchers to rapidly manipulate intracellular calcium levels in live-cell systems. This formulation enables time-efficient experimental setups in studies involving calcium signaling, apoptosis, immune response, and more.

With ≥98% purity (HPLC), this high-quality ionomycin solution is ideal for reproducible research outcomes, eliminating the need for labor-intensive compound solubilization and dilution.

Key Features and Benefits

• Ready-to-use formulation (1 mM in DMSO): Saves time and ensures accuracy in preparation
• High purity (≥98%): HPLC-verified for optimal performance in sensitive applications
• Efficient calcium carrier: Rapidly elevates intracellular Ca²⁺ levels in a controlled manner
• Versatile research tool: Useful in cell signaling, apoptosis, T cell activation, and muscle physiology
• Stable and convenient: Supplied in 1 mL aliquot; store at –20°C for up to 2 years

Applications

Ionomycin is widely used in:

• Cell signaling studies involving Ca²⁺-dependent pathways
• Apoptosis assays in cancer and neurobiology models
• T cell activation and immune cell function research
• Exosome release studies and protein kinase activation
• Muscle contraction and neurotransmitter release analysis
• Demyelination and ion channel regulation investigations

Biological Activity

• Mode of Action: Mobile ion carrier specific for Ca²⁺ > Mg²⁺ > Sr²⁺ = Ba²⁺
• Antimicrobial Spectrum: Effective against Gram-positive bacteria
• Mechanism: Binds and facilitates transport of Ca²⁺ and Pb²⁺ across membranes
• Effectiveness: More effective than A23187 for calcium mobilization

Specifications

Sigma-Aldrich Ionomycin Calcium Salt (1 mM in DMSO) is a highly pure, pre-formulated calcium ionophore solution designed for precise and reproducible manipulation of intracellular calcium levels in experimental systems. Its versatility across a wide range of biological applications makes it a critical reagent in cell biology, immunology, cancer research, and neuroscience.