Invitrogen™ ImaGene Green™ C12FDG lacZ Gene Expression Kit

Fluorescent β-galactosidase reporter assay system for sensitive lacZ expression detection

The Invitrogen™ ImaGene Green™ C12FDG lacZ Gene Expression Kit provides a high-sensitivity, fluorescence-based system for detecting β-galactosidase (lacZ) reporter gene expression in live cells. The kit’s core substrate, C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside), is a fluorescein-based β-galactosidase substrate covalently modified with a 12-carbon lipophilic tail to enhance cellular retention of the fluorescent product.

Once inside the cell, C12FDG is cleaved by β-galactosidase to produce a bright green fluorescent product (excitation/emission ~490/514 nm) that is stably retained, likely via incorporation of its lipophilic moiety into cellular membranes. The kit also includes PETG, a broad-spectrum β-galactosidase inhibitor, and chloroquine diphosphate to prevent acidic hydrolysis, ensuring specific and accurate reporter detection.

Key Features

• High Sensitivity – Detects even low levels of lacZ expression in mammalian cells
• Long-Term Signal Retention – Lipophilic product remains within cells for extended analysis
• Bright Green Fluorescence – Compatible with standard FITC/Alexa Fluor™ 488 filter sets
• Inhibitor-Enhanced Specificity – PETG and chloroquine reduce background signal
• Flexible Format – Suitable for live-cell imaging, flow cytometry, and microplate assays

Applications

• lacZ reporter gene assays in transfection and transduction experiments
• Live-cell β-galactosidase detection for gene expression analysis
• Flow cytometry-based lacZ activity quantification
• Screening assays in drug discovery and molecular biology research
• Gene regulation and promoter activity studies

Specifications

The ImaGene Green™ C12FDG lacZ Gene Expression Kit offers a sensitive, specific, and retention-optimized solution for detecting β-galactosidase reporter activity. With its bright, stable fluorescence and included reagents for signal optimization, it’s an ideal choice for researchers performing lacZ-based reporter assays in molecular and cell biology.