Gibco™ BenchStable™ DMEM/F12 with GlutaMAX, Phenol Red, No HEPES

Gibco™ BenchStable™ DMEM/F-12 is a next-generation formulation of Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 engineered to deliver consistent mammalian cell culture performance with the added operational advantage of room temperature storage.

This innovative medium supports cellular proliferation and maximum cell densities comparable to conventional refrigerated DMEM/F-12 when supplemented with ≥10% Fetal Bovine Serum (FBS), while significantly simplifying storage and logistics requirements.

BenchStable™ technology enables laboratories to maintain high culture standards without reliance on cold-chain storage, making it especially valuable in resource-conscious or high-throughput research environments.

Key Features

• Room Temperature Stable – No refrigeration required for storage or shipping
• GlutaMAX™ Technology – Minimizes ammonia accumulation and enhances cell viability
• High Glucose Content – Supports robust cell growth
• Sodium Pyruvate Included – Additional metabolic energy source
• Phenol Red Indicator – Convenient visual pH monitoring
• Animal Origin-Free
• cGMP Manufactured (ISO 9001:2015 compliant)
• ACT Ecolabel (Silver) Certified

Formulation Overview

DMEM/F-12 is a 1:1 combination of DMEM and Ham’s F-12, integrating:

• Elevated glucose, amino acids, and vitamins from DMEM
• A broad range of trace elements and nutrients from F-12

Included Additives

✔ High Glucose

✔ GlutaMAX™

✔ Phenol Red

✔ Sodium Pyruvate

Excluded Components

✘ No HEPES

✘ No proteins, lipids, or growth factors

Supplementation Requirement: ≥10% FBS for optimal growth performance.

Recommended Culture Conditions

• Buffer System: Sodium bicarbonate
• CO₂ Requirement: 5–10% CO₂ incubator
• Storage: Room temperature, protect from light
• Shelf Life: 12 months from date of manufacture
• Sterility: Sterile-filtered

Validated Cell Lines

Successfully used for culturing:

• MDCK cells
• Glial cells
• Fibroblasts
• Human endothelial cells
• Rat fibroblasts

Technical Specifications

Ordering Information

Unique Advantages

• Reduces cold-chain dependency
• Lowers storage and shipping costs
• Ideal for laboratories with limited refrigeration capacity
• Maintains performance consistency of conventional DMEM/F-12
• Enhances workflow flexibility in high-throughput settings

Gibco™ BenchStable™ DMEM/F-12 represents a strategic advancement in cell culture media technology, combining the trusted performance of traditional DMEM/F-12 with the practical benefits of ambient storage stability. By eliminating cold-chain constraints without compromising cell growth, viability, or reproducibility, this formulation delivers operational efficiency alongside scientific reliability.