Gibco™ Antibiotic-Antimycotic (100X), Penicillin–Streptomycin–Amphotericin B, Sterile-filtered

Gibco™ Antibiotic-Antimycotic (100X) is a sterile, ready-to-use antimicrobial supplement formulated to prevent bacterial and fungal contamination in mammalian cell culture. This solution contains a combination of penicillin, streptomycin, and Amphotericin B, providing broad-spectrum protection against common cell culture contaminants including Gram-positive bacteria, Gram-negative bacteria, yeast, and fungi.

It is widely used in routine cell culture workflows where maintaining aseptic conditions is critical.

Key Features

• Broad-spectrum antimicrobial protection for mammalian cell culture
• Effective against both Gram-positive and Gram-negative bacteria
• Includes Amphotericin B for antifungal and anti-yeast activity
• Sterile-filtered for direct addition to culture media
• Convenient 100X concentration for easy dilution
• Suitable for routine and long-term cell culture applications

Mode of Action

Penicillin

Penicillin acts by disrupting bacterial cell wall synthesis, interfering with cell wall turnover and triggering enzymatic breakdown of the wall structure.

Streptomycin

Streptomycin binds to the 30S ribosomal subunit, inhibiting protein synthesis and causing death in susceptible bacteria.

Amphotericin B

Amphotericin B prevents fungal and yeast growth by increasing plasma membrane permeability, leading to loss of cellular integrity.

Applications

• Routine mammalian cell culture maintenance
• Prevention of microbial contamination during cell handling
• Primary cell culture preparation
• Cell-based assays and research workflows
• General laboratory use requiring aseptic culture conditions

Available Pack Sizes

Technical Specifications

Storage & Handling

• Store at –5°C to –20°C
• Ship under dry ice conditions
• Avoid repeated freeze–thaw cycles for optimal performance

Regulatory Status

For Research Use Only. Not for use in diagnostic procedures.

Gibco™ Antibiotic-Antimycotic (100X) is a trusted and widely used supplement for safeguarding mammalian cell cultures against bacterial and fungal contamination. With its proven combination of penicillin, streptomycin, and Amphotericin B, it provides reliable broad-spectrum protection while supporting consistent, contamination-free research workflows. Its sterile, concentrated format ensures convenience, efficiency, and dependable performance in everyday cell culture applications.