Gibco™ TrypLE™ Express Enzyme (1X), phenol red

The Gibco™ TrypLE™ Express Enzyme (1X), Phenol Red, is a recombinant, animal origin-free (AOF) enzyme formulated for gentle and efficient dissociation of adherent mammalian cells. This enzyme is a direct replacement for trypsin and offers superior performance, reduced cell damage, and enhanced convenience for a wide range of cell culture applications.

Key Features:

1. Gentle on Cells:
   • Cleaves peptide bonds at the C-terminal sides of lysine and arginine with minimal damage, ensuring higher cell viability.
2. Room-Temperature Stable:
   • Stable for up to 24 months at room temperature (15–30°C), eliminating cold storage challenges.
3. Phenol Red Indicator:
   • Contains phenol red for pH monitoring during cell dissociation procedures.
4. Animal Origin-Free (AOF):
   • Free from animal-derived components, making it suitable for regulatory-compliant workflows and sensitive applications.
5. Ready-to-Use:
   • 1X liquid formulation allows direct use in standard protocols without dilution or additional preparation.
6. Self-Inactivating:
   • Dilution inactivates TrypLE Express, eliminating the need for trypsin inhibitors like FBS.

Specifications:

Applications:

1. Adherent Mammalian Cell Culture:
   • Efficiently dissociates various cell types, including CHO, HEK 293, A549, keratinocytes, and embryonic stem cells.
2. Primary Tissue Dissociation:
   • Ideal for breaking down tissues into single-cell suspensions for downstream applications.
3. Cell Passaging:
   • Provides a gentle solution for routine subculturing of sensitive adherent cells.
4. Phenol Red-Dependent Assays:
   • Suitable for workflows requiring a pH indicator for monitoring during dissociation.

Advantages:

1. High Purity and Specificity:
   • Reduces non-specific cleavage and potential damage caused by multiple enzymes in traditional trypsin.
2. Regulatory Compliance:
   • Manufactured under cGMP conditions at ISO 13485-certified facilities.
3. Easy Substitution:
   • Seamlessly replaces trypsin in existing protocols with no changes required.
4. Enhanced Workflow Efficiency:
   • Ready-to-use formulation minimizes preparation time and simplifies handling.
5. Eco-Friendly Manufacturing:
   • Produced using energy-efficient methods and sustainable packaging.

Protocol Overview:

1. Pre-Warm TrypLE:
   • Warm TrypLE Express to room temperature before use.
2. Prepare Cells:
   • Wash adherent cells with PBS to remove residual serum, which can inhibit the enzyme.
3. Apply TrypLE:
   • Add enough TrypLE Express to cover the cell monolayer.
4. Incubate:
   • Incubate at 37°C for 2–5 minutes, monitoring for cell detachment.
5. Neutralize:
   • Dilute with complete growth medium to inactivate TrypLE Express.
6. Centrifuge and Resuspend:
   • Centrifuge the cells and resuspend in fresh medium for further processing.

Ordering Information:

Storage and Handling:

• Primary Storage:
  • Store at 15°C to 30°C, protected from light.
• Alternate Storage:
  • Store at 2°C to 8°C or -5°C to -20°C for extended stability.
• Shelf Life:
  • 24 months from the date of manufacture.

Related Products:

• Gibco™ TrypLE Express (No Phenol Red): For workflows requiring phenol red-free dissociation.
• Gibco™ PBS (Calcium and Magnesium-Free): For washing cells before applying TrypLE.
• Gibco™ DMEM/F-12 Medium: For neutralizing and culturing cells post-dissociation.

The Gibco™ TrypLE™ Express Enzyme (1X), Phenol Red, is a versatile and reliable dissociation reagent for adherent mammalian cell cultures. Its animal origin-free composition, room-temperature stability, and superior performance make it an essential tool for cell culture workflows.