Gibco™ Pbs, Ph 7.4, 500 Ml

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Without Calcium, Magnesium, Phenol Red

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Specifications:
Application Cell culture
Storage Temperature Room Temperature
Product Type Buffer Forms Liquid
Product Brand Thermo Fisher Scientific™
Product Grade Cell Culture

Sterile, Balanced Salt Solution for Reliable and Reproducible Cell Culture Workflows

Gibco™ Phosphate Buffered Saline (PBS), pH 7.4 is a sterile, balanced salt solution formulated without calcium, magnesium, or phenol red. Manufactured to cGMP standards under ISO 13485 compliance, this high-quality buffer is specifically designed for a broad range of cell culture and molecular biology applications. It offers physiological pH and osmolality, ensuring compatibility with mammalian cells and minimizing cellular stress or experimental interference.

PBS, pH 7.4, is trusted globally by researchers for washing cells, preparing reagents, transporting samples, and diluting cell suspensions, providing a consistent and reliable solution for sensitive biological experiments.

Key Features & Benefits

  • Formulated Without Calcium and Magnesium
    Prevents unwanted cell adhesion and aggregation during cell dissociation, washing, or flow cytometry workflows.
  • Free of Phenol Red
    Eliminates interference in colorimetric assays or optical detection, and avoids potential cellular effects caused by phenol red.
  • Physiologically Relevant pH (7.4)
    Mimics the pH of human blood, supporting cell viability and stability during critical handling steps.
  • Animal-Origin-Free & Sterile
    Produced without animal-derived components and sterile-filtered to ensure product consistency, safety, and reproducibility.
  • Manufacturing Excellence
    Produced in ISO 13485–certified, cGMP-compliant facilities with rigorous lot release testing for pH, osmolality, sterility, and endotoxins.
  • Convenient & Stable Format
    Supplied as a 1X liquid solution, ready to use with a 24-month shelf life and room-temperature shipping for easier logistics and storage.

Applications

  • Cell Washing
    Rinse cells prior to enzymatic dissociation to remove residual media and divalent cations.
  • Tissue and Cell Transport
    Maintain sample viability during short-term handling or transport between processes.
  • Cell Counting
    Dilute cell suspensions accurately before loading onto hemocytometers or automated counters.
  • Reagent Preparation
    Serve as a physiological buffer base for antibody solutions, enzyme mixes, or sample dilutions.
  • Assay Support
    Use as a non-reactive medium for ELISAs, Western blots, and immunofluorescence protocols.

Specifications Table

AttributeDetails
Product NameGibco™ PBS, pH 7.4
Catalog Numbers10010023 (500 mL), 10010031 (1000 mL)
FormulationWithout calcium, magnesium, or phenol red
GradeMolecular Biology Grade, Animal Origin-Free
Format1X Liquid, ready to use
pH7.4 (range: 6.0 to 8.0)
Osmolality280 – 315 mOsm/kg
SterilitySterile-filtered
Storage Conditions15°C to 30°C
Shipping ConditionsRoom temperature
Shelf Life24 months from date of manufacture
PackagingPolyethylene bottle
Intended UseFor Research Use or Further Manufacturing Use Only

Why Choose Gibco™ PBS, pH 7.4?

  • Trusted Brand: Widely cited in peer-reviewed publications and globally recognized for consistent performance.
  • Superior Quality Control: Extensive lot testing ensures reliability in sensitive applications.
  • Research Versatility: Ideal for any lab performing mammalian cell culture, molecular biology, or immunological assays.
  • Simplicity and Compatibility: Preferred over more complex buffers like DPBS when additional ions or additives may interfere.


Gibco™ PBS, pH 7.4 provides a clean, consistent, and reliable buffer solution for cell culture and molecular biology workflows. Whether you're washing cells, preparing reagents, or supporting cell viability during handling, this PBS formulation delivers superior performance in every bottle.


  • Pack Size:   500 mL 1 LIter
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