Gibco™ Human IL-2 Recombinant Protein

The Gibco™ Human IL-2 Recombinant Protein is a highly purified cytokine designed for use in functional assays, such as T cell activation, proliferation, and immune response studies. This recombinant protein is expressed in E. coli and is highly active, supporting various immunological research applications.

Key Features:

1. High Purity:
   • Purity >95% as determined by SDS-PAGE.
2. Low Endotoxin Levels:
   • Endotoxin concentration: <0.1 ng/µg.
3. Biological Activity:
   • ED50 < 0.5 ng/mL, determined by the dose-dependent proliferation of mouse CTLL-2 cells.
4. Reconstitution Instructions:
   • Reconstitute in 100 mM acetic acid to a concentration of 0.1–1.0 mg/mL for full activity.
5. Versatile Use:
   • Designed for Western blot, ELISA, immunohistochemistry, and functional assays.

Specifications:

Applications:

1. T Cell Activation:
   • Stimulates proliferation and activation of T cells, including CD4+ and CD8+ subsets.
2. Cytokine Research:
   • Investigates the role of IL-2 in immune modulation and inflammation.
3. Cancer Research:
   • Studies the effect of IL-2 in enhancing the immune response against tumors.
4. Immunotherapy Development:
   • Supports preclinical research into IL-2-based therapies and immune checkpoint inhibitors.
5. In Vitro Assays:
   • Useful in Western blotting, ELISA, and proteomics for detecting IL-2-responsive pathways.

Protocol Overview:

1. Reconstitution:
   • Centrifuge the vial briefly before opening.
   • Reconstitute in 100 mM acetic acid to a concentration of 0.1–1.0 mg/mL.
2. Storage:
   • Store aliquots at ≤–20°C to avoid repeated freeze-thaw cycles.
3. Functional Assay:
   • Add reconstituted IL-2 to cell cultures for stimulation of immune responses, including T cell proliferation.

Ordering Information:

Storage and Handling:

• Lyophilized Form:
  • Store at 2–8°C, preferably in a desiccated environment.
• After Reconstitution:
  • Store at ≤–20°C in working aliquots to maintain activity and avoid freeze-thaw cycles.

Scientific Insights:

• Interleukin-2 (IL-2):
  • A cytokine crucial for T cell proliferation, B cell differentiation, and immune modulation.
  • Signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex.
  • Plays a critical role in immune responses to infection and antigenic stimuli.

Bioinformatics:

• Aliases:
  • Aldesleukin, T Cell Growth Factor (TCGF), IL-2.
• UniProt ID:
  • P60568.
• Entrez Gene ID:
  • 3558.

References:

1. Cancer Research:
   • Investigated the role of IL-2 in T cell activation for combating non-small cell lung cancer (Li et al., 2018).
2. Immunotherapy:
   • Studied IL-2 as an immune checkpoint inhibitor for melanoma and NSCLC therapies (Wang et al., 2021).

Related Products:

• Dynabeads™ Human T-Activator CD3/CD28: For T cell activation and expansion.
• CTS OpTmizer T Cell Expansion SFM: Serum-free medium for T cell culture.
• Human IL-2 ELISA Kits: For quantitative measurement of IL-2 in biological samples.

The Gibco™ Human IL-2 Recombinant Protein is a versatile, high-purity cytokine that supports cutting-edge research in immunology, cancer therapy, and cellular biology. Its robust activity and compatibility with various assays make it an essential tool for advancing immune response studies.