Gibco™ HEPES (1M) is a high-quality zwitterionic buffering agent widely used in cell culture systems that require stable pH control, especially during extended handling outside a CO₂ incubator. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) provides an effective buffering range around physiological pH and maintains environmental stability even under conditions where bicarbonate-buffered media may not perform optimally.

Gibco™ HEPES is manufactured under ISO 13485 and cGMP-compliant quality systems at two redundant facilities (Grand Island, USA and Scotland, UK) to ensure supply continuity and consistent performance in research and bioprocessing applications.

Catalog Information

Key Features

• Provides extra buffering capacity at 10–25 mM when media is used outside CO₂ incubators.
• Zwitterionic buffer with minimal effects on cellular processes and biochemical reactions.
• Membrane-impermeable, ensuring stable extracellular buffering.
• Chemically and enzymatically stable across a wide range of conditions.
• Low absorbance in visible and UV ranges, making it suitable for optical assays.
• Sterile-filtered liquid formulation ready for direct use in cell culture workflows.
• Backed by dual-site manufacturing for supply chain reliability.

Applications

Gibco™ HEPES (1M) is widely used in:

• Mammalian cell culture requiring pH stability
• Procedures conducted outside incubators (microscopy, manipulation, sorting)
• Biochemical and molecular biology workflows
• Chromatin biology and nucleic acid research
• Assays sensitive to bicarbonate/CO₂ fluctuations

Specifications

Gibco™ HEPES (1M) is a high-quality, reliable buffering solution ideal for cell culture systems requiring enhanced pH stability, especially during operations outside controlled CO₂ conditions. With dual cGMP-compliant manufacturing sites, exceptional chemical stability, and low optical interference, this HEPES buffer offers consistency and performance trusted by researchers across the biological and biochemical sciences.