GeneJET™ Plasmid Maxiprep Kit

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture

• High concentration of DNA purified— >400 ng/μL

• Convenient—spin column format, centrifuge or vacuum protocols

• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

• Transfection of robust cell lines

• FastDigest restriction enzyme digestion

• Automated fluorescent sequencing

• PCR

• Cloning

• Transformation

• In vitro transcription