Vircell Respiratory Syncytial Virus Elisa Kit IgG/IgM

The Vircell Respiratory Syncytial Virus (RSV) ELISA Kit IgG/IgM is a high-performance indirect immunoenzyme assay designed for the detection of IgG and IgM antibodies against RSV in human serum or plasma. This kit provides a reliable and user-friendly solution for diagnosing RSV infections, suitable for manual and automated ELISA systems.

Key Features

1. IgG/IgM Dual Detection:
   • Enables the differentiation between acute and past RSV infections by measuring both IgG and IgM antibodies.
2. Simplified Sample Preparation:
   • Serum dilution and sorbent treatment, if required, are performed directly in the well without pre-dilution.
3. Automation Compatible:
   • Fully compatible with automated ELISA systems, facilitating high-throughput testing.
4. Ready-to-Use Reagents:
   • Pre-colored, liquid reagents reduce handling errors and improve workflow efficiency.
5. Enhanced Design:
   • Break-apart, color-coded wells allow flexible use and accurate testing for various sample sizes.
6. Stable Reagents:
   • Lyophilized conjugates (if needed) ensure long-term stability and consistent performance.

Principle of the Assay

The Vircell RSV ELISA Kit employs an indirect ELISA methodology:

1. Antigen Binding:
   • RSV antigens are adsorbed onto the surface of the polystyrene wells.
2. Sample Interaction:
   • Antibodies in the sample bind to the adsorbed RSV antigens, while unbound immunoglobulins are washed off.
3. Conjugate Reaction:
   • An enzyme-labeled anti-human globulin binds to the antigen-antibody complex.
4. Signal Development:
   • TMB substrate is added, producing a blue-colored solution. The color turns yellow upon adding the stop solution.
5. Quantification:
   • The optical density (OD) is measured at 450 nm, and antibody levels are determined based on OD values.

Applications and Use Cases

1. Clinical Diagnostics:

• Acute RSV Infection Detection:
  • Detect IgM antibodies for early-phase RSV infections.
• Immunity Assessment:
  • Measure IgG antibodies to evaluate immunity from past infections or vaccination.

2. Epidemiological Studies:

• Seroprevalence Monitoring:
  • Assess RSV exposure in different populations.
• Outbreak Investigation:
  • Identify infection patterns during RSV outbreaks.

3. Vaccine Research:

• Vaccine Response Monitoring:
  • Evaluate antibody production post-vaccination.
• Efficacy Studies:
  • Assess the immunogenicity of RSV vaccines.

4. Laboratory Research:

• Viral Immunology Studies:
  • Explore immune responses to RSV infections.
• RSV-Specific Antibody Profiling:
  • Differentiate IgG and IgM responses in RSV infections.

Kit Components

Assay Specifications

Advantages

1. Dual Detection Capability:
   • Detects both IgG and IgM antibodies for a comprehensive serological profile.
2. Efficient Workflow:
   • Ready-to-use reagents and direct in-well sample processing save time and reduce errors.
3. Flexible Testing:
   • Break-apart wells accommodate variable sample sizes and batch testing.
4. High Sensitivity and Specificity:
   • Accurate detection of RSV-specific antibodies with minimal cross-reactivity.
5. Automation-Ready:
   • Compatible with automated systems, enabling high-throughput diagnostics.

Storage and Stability

• Storage Conditions: Store all components at 2–8°C.
• Shelf Life: Use before the expiration date indicated on the product label.

The Vircell Respiratory Syncytial Virus (RSV) ELISA Kit IgG/IgM is a reliable and efficient solution for detecting RSV-specific antibodies in human serum and plasma. Its dual detection capability, automation compatibility, and ready-to-use reagents make it ideal for clinical diagnostics, vaccine research, and epidemiological studies.