NANOPORE Technologies SFB Expansion

The SFB Expansion provides additional Short Fragment Buffer (SFB) to support sequencing experiments that require shorter DNA fragments. It is particularly recommended for workflows like the PCR Tiling of COVID-19 Virus Protocol or applications prioritizing shorter fragment sequencing.

Key Features

1. Short Fragment Buffer for Specialized Applications:
   • Designed for sequencing experiments where short DNA fragments are required.
   • Essential for specific protocols like the PCR tiling of SARS-CoV-2.
2. Compatibility:
   • Supports a wide range of ONT kits and devices, including:
     • Ligation Sequencing Kits: SQK-LSK109, SQK-LSK109-XL, SQK-LSK110, and V14 versions (SQK-LSK114, SQK-LSK114-XL).
     • PCR and Native Barcoding Expansions: For various multiplexing needs (1–12, 13–24, 96 barcodes).
   • Compatible with all flow cells and sequencing devices (MinION Mk1B, MinION Mk1C, GridION, and PromethION).
3. Pack Size and Formats:
   • Available in two formats:
     • Vial Format: Four vials, each containing 1,800 μL of Short Fragment Buffer.
     • Bottle Format: One bottle with 7,500 μL of Short Fragment Buffer.
4. Ease of Integration:
   • Integrates seamlessly into ONT workflows, enhancing versatility for custom sequencing needs.

Stability and Storage

• Shipping Conditions:
  • Shipped at 2–8°C in temperature-controlled packaging.
  • Environmentally friendly shipping options available.
• Storage Conditions:
  • Long-term storage at -20°C.
  • Product lifespan is deemed to be 3 months from customer receipt.

Workflow and Usage

1. Buffer Application:
   • Used to prepare DNA libraries for short-fragment-focused sequencing experiments.
2. Applications:
   • Ideal for tiled PCR applications like COVID-19 genome sequencing.
   • Can be incorporated into workflows requiring shorter fragment enrichment.
3. Flexibility:
   • Compatible with other sequencing kits for tailored workflows and diverse experimental requirements.

What’s in the Box

Associated Kits

The SFB Expansion is compatible with the following kits:

• Ligation Sequencing Kits: Regular (SQK-LSK109/110) and V14 versions (SQK-LSK114).
• PCR Barcoding Expansions: 1–12 (EXP-PBC001) and 1–96 (EXP-PBC096).
• Native Barcoding Expansions: 1–12 (EXP-NBD104), 13–24 (EXP-NBD114), and 96 (EXP-NBD196), including V14 versions.

Device and Flow Cell Compatibility

• Works with all ONT flow cells and sequencing platforms:
  • Devices: MinION Mk1B, MinION Mk1C, GridION, PromethION.
  • Flow Cells: All types, ensuring adaptability to user needs.

Safety and Legal Notes

• Safety Data Sheets available for proper handling.
• Not intended for clinical diagnostics or disease treatment.

The SFB Expansion is a critical addition to sequencing workflows requiring short DNA fragments, offering flexibility, compatibility, and convenience across various ONT sequencing platforms and kits. Its specialized formulation supports enhanced performance in targeted applications, such as pathogen surveillance and genome tiling studies.